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Development of Versatile Environmental Cell for Electron Microscope

电子显微镜多功能环境池的研制

基本信息

批准号：
16560025
负责人：
IHSIKAWA Akira
金额：
$ 2.05万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

The closed type environmental cell (EC) has been put into practical use for observing the hydrated specimens with the standard electron microscopes. Based on this design of the closed type cell, the film-sealed type EC for versatile use has been developed.(1)Improvement of the closed type EC and confirmation of the estimation of the gas pressure in the ECThe closed type EC was newly designed to control the gas layer thickness from 0 to 0.2mm for observation with high resolution and for holding very thin liquid layer. For the closed type EC, the sealing film is the most important component of the cell because it must withstand the pressure higher than the atmospheric pressure when the EC is closed in the atmospheric condition. We developed the method for the best sealing film with high withstanding pressure and high electron transmittance. We also made the device for testing the withstanding pressure of the sealing films for improving the efficiency of the EC experiment.For high resolut … More ion observations, a pre-evacuation unit was made to set the gas pressure lower than the atmospheric pressure before sealing up the cell. The thickness of the gas layer in the cell can be estimated from the transmittance value plotted as the function of the mass thickness of the electron path, involving two sealing films and the gas layer. This method may be applied to estimate the thickness of the liquid layer in the cell for observing the biological specimens in the nearly natural environment.(2)Development of the film-sealed type environmental cell for versatile use and the gas control unitFor the practical use, the configuration of the film sealed type EC and the gas pressure control system were improved. By reducing the components of the EC, it became easy to assemble and handle the EC. In addition to this, the sealing film of high withstanding pressure could make the procedure of controlling the gas pressure in the EC simple, so we made a new gas control device to make the operation easy.For versatile use of the EC, small electrode unit was made and fitted into the EC. The pipes for gas circulation can be used as the passages for the conducting wires and some electric field can be made in the EC. With the use of the electrode unit, it was confirmed that the ions, produced by the electron irradiation to the gas molecules in the EC, can be collected efficiently.This technique can set not only the gas environment, but also some liquid environment in the EC and the thickness of the liquid layer can be controlled by balancing the speed between supplying and evacuating the gas in the EC. This technique must be useful tool for in-situ observation of hydrated specimens. Less

封闭式环境池（EC）已投入实际使用，用于用标准电子显微镜观察水合样品。基于这种密闭型样品池的设计，开发了多功能的薄膜密封型EC。(1)密闭型EC的改进和EC内气压估计的确认密闭型EC经过新设计，可将气层厚度控制在0至0.2mm之间，以进行高分辨率观察并保持非常薄的液体层。对于封闭型EC来说，密封膜是电池最重要的组成部分，因为当EC在大气条件下封闭时，密封膜必须承受高于大气压的压力。我们开发了具有高耐压和高电子透过率的最佳密封膜的方法。我们还制作了密封膜耐压测试装置，以提高EC实验的效率。为了进行高分辨率离子观察，我们制作了预抽气装置，在密封电池之前将气体压力设置为低于大气压。电池中气体层的厚度可以根据作为电子路径质量厚度的函数绘制的透射率值来估计，涉及两个密封膜和气体层。该方法可用于估计细胞内液层的厚度，以在接近自然的环境中观察生物标本。(2)多功能用途薄膜密封型环境细胞和气体控制单元的开发针对实际应用，改进了薄膜密封型EC和气压控制系统的配置。通过减少 EC 的组件，EC 的组装和操作变得容易。除此之外，高耐压的密封膜可以使控制EC内气压的过程变得简单，因此我们制作了一种新的气体控制装置，使操作变得容易。为了EC的多功能使用，制作了小型电极单元并将其安装在EC中。气体循环的管道可以作为导线的通道，并且可以在EC中产生一定的电场。通过使用电极单元，证实可以有效地收集电子照射EC中的气体分子所产生的离子。该技术不仅可以设置EC中的气体环境，还可以设置EC中的一些液体环境，并且可以通过平衡EC中的气体供应和排出之间的速度来控制液体层的厚度。该技术必定是水合标本原位观察的有用工具。较少的