Isotypes of multilocalizing proteins from the cytoskeleton and their roles in differentiation and growth of pea seedlings.

细胞骨架多定位蛋白的同种型及其在豌豆幼苗分化和生长中的作用。

• 批准号: 16570034
• 负责人: ABE Shunnosuke
• 金额: $ 2.05万
• 依托单位: Ehime University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16570034/
• 关键词: apyrase; Tudor; differentiation; Lateral root formation; sub-cellular localization; immunohistochemistry; translation; cytoskeleton; 4SNc-Tudor; 発芽; マメ類; 誘導酵素; アピラーゼ; SN4TDR; 吸水; 遺伝子発現; タンパク質; 分裂酵母

Expression, localization, and molecular structure of the heparin binding protein SN4TDR and apy1 from the pea cytoskeleton were studied. Both SN4TDR and apy1 were localized in the cytoplasm in pea seedling tissues and also induced in the primodium for lateral root formation. In some active cells, apy1 was also localized in nuclei. However, expression of SN4TDR was induced during imbibition and kept constant after germination as in tub1, while apy1 was induced 16 h after germination. Localization of these proteins in the cytoplasm were proved by immunohistochemistry in pea seedlings. When with the full SN4TDR was fused with XFP and expressed in S.pombe, the protein was proved to localize in cytoplasm, leaving nuclei unstained throughout the life cycle (vegetative, mating, and sporulation. Deletion experiments of the YFP construct in S.pombe implied the presence of nuclear exclusion signal (NES) with less than 50 aa long in SN4TDR. Yeast two hybridization followed by immunoprecipitation.proposed the tubulin binding subunit of translation initiation factor, moe1 and ribosomal protein L22 potent as candidates for association of SN4TDR in vivo. Phosphorylation of apy1 was also proved.

研究了豌豆细胞骨架中肝素结合蛋白SN4TDR和apy1的表达、定位和分子结构。SN4TDR和apy1都定位于豌豆幼苗组织的细胞质中，并且在侧根形成的原基中也被诱导。在一些活跃的细胞中，apy 1也定位于细胞核。然而，SN4TDR的表达诱导吸胀过程中，并保持恒定的萌发后，在tub1，而apy1诱导萌发后16小时。这些蛋白质的定位在豌豆幼苗的细胞质中的免疫组织化学证明。当完整的SN4TDR与XFP融合并在粟酒裂殖酵母中表达时，证明该蛋白定位于细胞质中，在整个生命周期（营养、交配和孢子形成）中留下核不染色。在粟酒裂殖酵母中YFP构建体的缺失实验暗示在SN4 TDR中存在长度小于50个aa的核排斥信号（内斯）。酵母双杂交后免疫沉淀，提出了翻译起始因子微管蛋白结合亚基moe1和核糖体蛋白L22作为SN4TDR在体内结合的候选者。还证实了apy1的磷酸化。

项目成果

Co-expression of an ethylene receptor gene, ERS1, and ethylene signaling regulator gene, CTR1, in Delphinium during abscission of florets.

• DOI: 10.1016/j.plaphy.2004.07.006
• 发表时间: 2004-09
• 期刊: Plant physiology and biochemistry : PPB
• 作者: S. Kuroda;Yukio Hirose;M. Shiraishi;E. Davies;S. Abe

Role of 4SNc-Tudor domain protein in protein synthesis in plants : functional analysis using a model system, Schizosaccharomyces pombe

4SNc-Tudor 结构域蛋白在植物蛋白质合成中的作用：使用模型系统裂殖酵母进行功能分析

• 发表时间: 2005
• 期刊: Supplement to Plant Physiology : Abstract for Plant Biology 2005 July 16-20,2005,Seattle, Washington, USA
• 作者: Awata;S.;Heg;D.;Munehara;H.;Kohda;M.;Shunnosuke Abe
• 通讯作者: Shunnosuke Abe

Antioxidant Activity of Triticale Caryopses and Embryos Extracts

小黑麦颖果和胚提取物的抗氧化活性

• 发表时间: 2004
• 期刊: Food Science and Biotechnology
• 影响因子: 2.9
• 作者: Ota K;Kohda M;T.Konishi et al.;阿部俊之助ほか
• 通讯作者: 阿部俊之助ほか

Isolation and identification of 3-methylcrotonyl CoA carboxylase cDNAs and pyruvate carboxylase, and their expression in red seabream, Pagrus major organs

3-甲基巴豆酰辅酶A羧化酶cDNA和丙酮酸羧化酶的分离鉴定及其在真鲷、真鲷主要器官中的表达

• 发表时间: 2005
• 期刊: Marine Biotechnology 6(6)
• 作者: Shunnosuke Abe;Chhoun Chamnan;Kenichi Miyamoto;Yasutaka Minamino;Makoto Nouda
• 通讯作者: Makoto Nouda

Expression of apyrase(APY1) and Tudor protein(SN4TDR) during early stages of germination of peas(Pisum sativum L.var.Alaska) in the dark

豌豆(Pisum sativum L.var.Alaska)黑暗萌发早期腺苷三磷酸双磷酸酶(APY1)和Tudor蛋白(SN4TDR)的表达

• 发表时间: 2005
• 期刊: Supplement to Plant Physiology : Abstract for Plant Biology 2005 July 16-20,2005, Seattle, Washington, USA
• 作者: Awata S;Heg D;Munehara H;Kohda M;Shunnosuke Abe
• 通讯作者: Shunnosuke Abe