Identification and characterization of genes for cell polarity and asymmetric cell division in plants

植物细胞极性和不对称细胞分裂基因的鉴定和表征

• 批准号: 16570041
• 负责人: FUJITA Tomomichi
• 金额: $ 2.43万
• 依托单位: Hokkaido University (2005)National Institute for Basic Biology (2004)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16570041/
• 关键词: plants; Physcomitrella patens; asymmetric cell division; gene expression; development; cell differentiation; cell polarity; stem cell; 不等分裂; ヒメツリガネゴケ; プロトプラスト; 完全長cDNA; 過剰発現; RNA干渉; 再生

Asymmetric cell division generates two different daughter cells. Although unequal distribution of mRNA or proteins has been known to play a pivotal role to specialize each daughter cell, such molecules in plants remain largely unknown. The moss, Physcomitrella patens assures a good system for the study of molecular mechanisms for asymmetric cell division. The protoplast divides asymmetrically to generate apical stem cell and differentiated protonemal, non-stem cell, thereafter the apical stem cell continues to divide asymmetrically to generate a row of differentiated protonemal cells. We have devised a systematic overexpression screening and identified 58 cDNAs as candidates for genes that are involved in asymmetric cell division. For those candidates, we made cDNA-citrine knock-in transgenic plants by using gene targeting technique to investigate protein localization during asymmetric cell division under a control of native promoters. We found several fusion proteins preferentially accumulated in apical stem cells, two of which were intracellularly localized within the apical cell.

不对称的细胞分裂产生两个不同的子细胞。尽管已知mRNA或蛋白质的不均匀分布在每个子细胞的特化中发挥着关键作用，但植物中的此类分子在很大程度上仍然未知。小立碗藓为研究细胞不对称分裂的分子机制提供了一个良好的系统。原生质体不对称分裂产生顶端干细胞和分化的原丝体非干细胞，然后顶端干细胞继续不对称分裂产生一排分化的原丝体细胞。我们设计了一个系统的过表达筛选，并确定了58个cDNA作为参与不对称细胞分裂的基因的候选者。对于这些候选者，我们通过使用基因打靶技术构建cDNA-citrine敲入转基因植物，以研究在天然启动子控制下的不对称细胞分裂过程中的蛋白质定位。我们发现了几种融合蛋白优先积累在顶端干细胞，其中两个是细胞内定位在顶端细胞。

项目成果

Genes for the peptidoglycan synthesis pathway are essential for chloroplast division in moss

• DOI: 10.1073/pnas.0510693103
• 发表时间: 2006-04-25
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Machida, M;Takechi, K;Takano, H
• 通讯作者: Takano, H

Isolation of mutant lines with decreased numbers of chloroplasts per cell from a tagged mutant library of the moss Physcomitrella patens

• DOI: 10.1055/s-2005-837691
• 发表时间: 2005-05-01
• 期刊: PLANT BIOLOGY
• 影响因子: 3.9
• 作者: Hayashida, A;Takechi, K;Takano, H
• 通讯作者: Takano, H

New Frontiers in Bryology : Physiology, Molecular Biology and Functional Genomics

苔藓学新领域：生理学、分子生物学和功能基因组学

• 发表时间: 2004
• 作者: Fujita;T
• 通讯作者: T