权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

The 2-dimensional to 3-dimensional growth transition in Physcomitrella patens

小立碗藓 (Physcomitrella patens) 的 2 维到 3 维生长转变

基本信息

批准号：
2270227
负责人：
金额：
--
依托单位：
University of Oxford
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2019
资助国家：
英国
起止时间：
2019 至无数据
项目状态：
已结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2270227
关键词：
dimensional growth transition Physcomitrella patens

项目摘要

An important event in evolutionary history was the colonisation of the land by plants. In the charophyte green algae, the sister group of land plants, growth occurs from apical initial cells with only one or two cutting faces, producing simple forms. The movement from water to land was accompanied by the acquisition of apical initial cells that could cleave in three planes. 3-dimensional (3D) growth is thus a defining feature of all land plants, which underpins structurally complex biomes that form the foundation of important terrestrial ecosystems. The onset of 3D growth in flowering plants occurs during embryogenesis. For this reason, flowering plants such a Arabidopsis thaliana cannot easily be exploited to investigate the genetic basis of 3D growth. However, it is possible to genetically dissect 3D growth in the model moss Physcomitrella patens, an extant representative of one of the earliest diverging land plant lineages. In P. patens, a 2-dimensional (2D) growth phase precedes 3D growth initiation and this 2D phase can be maintained indefinitely. Consequently, forward genetics can be used to create viable 3D-defective mutants in P. patens and a somatic hybidisation based approach can then be used to identify causative mutations, and help elucidate the underlying regulatory networks underpinning 3D growth in plants. It was previously shown that the NO GAMETOPHORES 1 (PpNOG1) gene is essential for 3D growth in P. patens. The aim of this project is to perform further functional characterisation of PpNOG1 in P. patens, and through a comparative approach, in A. thaliana. The first strand of the project will determine the PpNOG1 interaction partners. Next, the function of NOG1 in A. thaliana will be explored. Additionally, the project will examine the effect of PpNOG1 on cell division planes and investigate phytohormone signalling during the transition to 3D growth. Finally, transcriptomics will be used to glean a full picture of how the 3D-defective mutants Ppnog1 & 2 differ transcriptionally from wild type.

进化史上的一个重要事件是植物对土地的殖民。轮生绿藻是陆地植物的姊妹类，它的生长始于顶端的初始细胞，只有一个或两个切面，形成简单的形式。从水到陆地的运动伴随着顶端初始细胞的获得，这些细胞可以分裂成三个平面。因此，三维(3D)生长是所有陆地植物的一个决定性特征，它支撑着构成重要陆地生态系统基础的结构复杂的生物群。开花植物的3D生长发生在胚胎发生期间。出于这个原因，像拟南芥这样的开花植物不容易被利用来研究3D生长的遗传基础。然而，有可能从基因上剖析模式苔藓Physcomitrella patens的3D生长，它是现存的最早分化的陆地植物谱系之一的代表。在P.patens中，二维(2D)生长阶段先于3D生长开始，并且这个2D阶段可以无限期地保持。因此，正向遗传学可以用来在P.patens中创建可行的3D缺陷突变体，然后可以使用基于体细胞杂交的方法来识别导致突变的原因，并帮助阐明支撑植物3D生长的潜在调控网络。以往的研究表明，NO GAMETOPHORES 1(PpNOG1)基因是光肩星天牛3D生长所必需的。本项目的目的是通过比较的方法，在P.patens和A.thaliana中进一步研究PpNOG1的功能特征。该项目的第一条线索将确定PpNOG1交互伙伴。接下来，我们将探讨NOG1在拟南芥中的功能。此外，该项目还将研究PpNOG1对细胞分裂平面的影响，并研究向3D生长过渡期间的植物激素信号。最后，转录组学将被用来收集3D缺陷突变体Ppnog1和2在转录上如何与野生型不同的全貌。