Characterization of genes for cell polarity and asymmetric cell division in plants

植物细胞极性和不对称细胞分裂基因的表征

• 批准号: 18570031
• 负责人: FUJITA Tomomichi
• 金额: $ 2.59万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18570031/
• 关键词: plants; cell division; gene; cell differentiation; cell polarity; protein; 発生; 発現制御

Asymmetric cell division produces two different daughter cells. Although unequal distribution of mRNA or proteins has been known to play a pivotal role to specialize each daughter cell, such molecules in plants remain largely unknown. Protoplasts and apical stem cells of protonemata of the moss, Physcomitrella patens divide asymmetrically to generate two different daughter cells. We have previously reported 59 genes as candidates involved in the asymmetric cell division. For those candidates, we made transgenic plants expressing the citrine-fusion protein by using the gene targeting technique to investigate protein localization under a control of their native promoters. We carried out time-lapse imaging of these proteins during asymmetric cell division and found some proteins accumulated preferentially in the stem cells but not in differentiated protonemal cells. We also showed some of them resumed the accumulation upon dedifferentiation of the protonemal, somatic cells.

不对称的细胞分裂产生两个不同的子细胞。尽管已知mRNA或蛋白质的不均匀分布在每个子细胞的特化中发挥着关键作用，但植物中的此类分子在很大程度上仍然未知。小立碗藓原丝体的原生质体和顶端干细胞不对称分裂，产生两个不同的子细胞。我们以前已经报道了59个基因作为候选人参与不对称细胞分裂。对于这些候选者，我们通过使用基因打靶技术来研究在其天然启动子控制下的蛋白质定位，从而制备表达柑橘碱融合蛋白的转基因植物。我们对这些蛋白质在不对称细胞分裂过程中进行了延时成像，发现一些蛋白质优先在干细胞中积累，而不是在分化的原丝体细胞中积累。我们还发现，其中一些恢复积累后，原丝体细胞的脱分化。

项目成果

植物細胞工学シリーズ23、植物の進化

植物细胞工程系列 23，植物进化

• 发表时间: 2007
• 作者: Fujita;T.;長谷部光泰
• 通讯作者: 長谷部光泰

Identification of proteins that accumulate preferentially in stem cells of Physcomitrella patens during their asymmetric cell division

鉴定小立碗藓干细胞在不对称细胞分裂过程中优先积累的蛋白质

• 发表时间: 2007
• 作者: Fujita;T.
• 通讯作者: T.

Involvement of mitochondrial-targeted RecA in the repair of mitochondrial DNA in the moss, Physcomitrella patens

• DOI: 10.1266/ggs.82.43
• 发表时间: 2007-02-01
• 期刊: GENES & GENETIC SYSTEMS
• 影响因子: 1.1
• 作者: Odahara, Masaki;Inouye, Takayuki;Sekine, Yasuhiko
• 通讯作者: Sekine, Yasuhiko

植物幹細胞の不等分裂により非対称に分配されるタンパク質の同定

植物干细胞不等分裂过程中不对称分布蛋白质的鉴定

• 发表时间: 2007
• 作者: Fujita;T.;藤田知道
• 通讯作者: 藤田知道

Genes for the peptidoglycan synthesis pathway are essential for chloroplast division in moss

• DOI: 10.1073/pnas.0510693103
• 发表时间: 2006-04-25
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Machida, M;Takechi, K;Takano, H
• 通讯作者: Takano, H