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Mobility and application for molecular breeding of transposon-like gene Revolver aimed genetic plant development

转座子样基因分子育种的移动性和应用 Revolver 旨在遗传植物开发

基本信息

批准号：
16580004
负责人：
TOMITA Motonori
金额：
$ 2.43万
依托单位：
Tottori University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

项目摘要

Revolver is a new class of transposon-like gene (US-2005-0091710) discovered in the Triticeae genome. Revolver encompassing 3041 bp has 20 bp of terminal inverted repeated sequence on the both ends, which features a class II transposable element. Revolver is transcriptionally active and its cDNA clone (726 bp) contains a single ORF encoding 118 amino acid residues of protein, which shows similarity with transcriptional regulators with DNA binding ability. The entire structure of Revolver dose not share identity with both the class I and class II transposable elements ever found. Southern blot analysis on the Triticeae plants shows that Revolver is abundant in the genomes of some wheat relatives, Secale (RR), Dasypyrum (VV), as well as wheat ancestor species, Triticum monococcum (AA), T.turgidum (AABB) and T.tauschii (DD). However, Revolver is rare in the genome of bread wheat T.aestivum (AABBDD). These facts indicate that Revolver has been existed since the diploid progenitor of wheat, then it has been disappearing in bread wheat through the evolution. Quantity of Revolver in wild emmer wheat Triticum dicoccoides, which is the tetraploid progenitor of bread wheat, was examined in 17 populations (209 genotypes) representing a wide range of ecological conditions of soil, temperature and water availability in Israel. Noticeable quantitative differences in copy numbers of Revolver was observed among the populations. T.dicoccoides in the population of Yehudiyya, Ammaid, Tabigha and Mt.Gerizim, where humid and temperate, had the highest number of copies (20,000) per haploid genome and that in the population of Kokhav-Hashahar, J'aba, Amirim, Beito-Oren, Bat-Shelomo, where seasonal hot and dry wind occurs over 85 days per year, had the lowest number (50 to 100). This extensive quantitative diversity among the locations suggests mobilization of Revolver by ecological stresses.

Revolver是在小麦基因组中发现的一类新的类转座子基因(US-2005-0091710)。包含3041个碱基对的左轮手枪在两端有20个碱基的末端反向重复序列，其特征是具有一个II类转座元件。Revorver具有转录活性，其cDNA克隆(726bp)包含一个编码118个氨基酸残基的ORF，这与具有DNA结合能力的转录调控因子相似。Revolver的整个结构与已发现的I类和II类转座元件不同。Southern杂交分析表明，Revolver在小麦近缘种Secale(RR)、Dasypyrum(VV)以及小麦祖先种Triticum monoccum(AA)、T.turgium(AABB)和T.tauschii(DD)的基因组中都含有丰富的Revolver。然而，在面包小麦T.aestivum(AABBDD)的基因组中，Revolver是罕见的。这些事实表明，自小麦二倍体祖先产生以来，Revolver就一直存在，并在进化过程中逐渐消失在面包小麦中。对四倍体面包小麦的四倍体祖先--野生二粒小麦的旋转量在17个群体(209个基因型)中进行了检测，这些群体代表了以色列广泛的土壤、温度和水分可利用性的生态条件。在种群间观察到了Revolver基因拷贝数的显著数量差异。湿润温带的耶胡迪亚、阿迈德、塔比阿和杰里津山种群的双孢子虫单倍体基因组拷贝数最高(20000)，而季节干热风年超过85天的Kokhav-Hashahar、J‘aba、Amirim、Beito-Oren、Bat-Shelomo种群的单倍体基因组拷贝数最低(50-100)。这些地点之间的这种广泛的数量差异表明，生态胁迫调动了旋转器。