Evolutional divergence and functional non-coding RNA analysis of transposon-like gene

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Evolutional mobilization of the transposon Revolver was analyzed by using wild-type barley adapted on the warm-dry and cool-wet slopes of “Evolution Canyon” in Israel. A 0.7-kb RNA molecule was highly transcribed in the wild-type barleyon the warm-dry slope, whereas various RNA molecules of 1-2 kb were transcribed at low levels in the barley on the cold-wet slope, indicating drastic variation due to the ecological environment and recent movable activity of Revolver. Noncoding RNA variants of Revolver with, for example, a long or short first exon and a deletion of the second intron were isolated. The transcription initiation site, the protein synthesized in E. coli, and the chromosomal gene cluster of Revolver were clarified.