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STRUCTURE, FUNCTION AND CHROMOSOME LOCATION OF THE NOVEL MULTIGENE FAMILY IN THE GENOME OF TRITICEAE SPECIES

小麦科物种基因组中新多基因家族的结构、功能和染色体位置

基本信息

批准号：
13660006
负责人：
TOMITA Motonori
金额：
$ 1.98万
依托单位：
TOTTORI UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2001
资助国家：
日本
起止时间：
2001 至 2002
项目状态：
已结题

项目摘要

A new class of transposable element with 3 kb length have been identified in the Triticeae genome. The transposon contains a single ORF of a transposase encoding 118 amino acid residues, which is conferred by a processed mRNA (726 nt). Firstly, a partial DNA fragment (89 bp) of the transposon enriched in the rye genome was isolated via the genomic subtraction of common DNA sequences between rye and wheat. Then, the whole structure of the rye genome-specific element is determined by sequencing genomic clones, which include the 89 bp segment. The consensus sequence of the element encompasses 3041 bp and it exists dispersedly in the rye genome by flanking with 20 bp of terminal inverted repeated sequence in the both ends. The transposon-like element is actively transcribed by Northern analysis. Moreover, cDNA clones (726 bp) derived from the transposon has identified in Triticeae plants, elucidating that the transposon bears 3 exons (342 bp, 88 bp, 296 bp) and 2 introns (750 bp, 1,237 bp). The entire structure of the transposon dose not share identity with known transposable elements, then the transposon is designated as Revolver. The deduced gene of Revolver encodes a protein featuring transcriptional regulators, having DNA binding ability. Genomic Southern blots on the Triticeae plants revearls that Revolver is abundant in the genomes of wheat relatives, rye, Dasypyrum, as well as wheat ancestor species, T.monococcum and Ae. squarrosa. At least ten thousand copies of Revolver are distributed along with each chromosome of wheat relatives. However, Revolver is quite rare in bread wheat varieties. These facts indicates that Revolver have been existed through the evolution since the ancestor of wheat, then it has been amplifing in some species, while disappearing in bread wheat. A new class of transposon-like gene Revolver is effective to develop DNA markers for wild wheat relatives, useful gene sources of wheat, and to evaluate phylogenetic evolution among the Triticeae

在小麦基因组中发现了一类新的长度为3kb的转座元件。转座子包含一个转座酶的ORF，编码118个氨基酸残基，由一个经过加工的mRNA(726个核苷酸)赋予。首先，通过对黑麦和小麦之间的共同DNA序列进行基因组消减，获得了富含在黑麦基因组中的转座子的部分DNA片段(89bp)。然后，通过对包括89bp片段的基因组克隆进行测序，确定了黑麦基因组特异元件的整个结构。该元件的共同序列全长3041bp，在黑麦基因组中以两端20bp的末端反向重复序列的形式分散存在。Northern分析表明，转座子样元件被激活转录。此外，从该转座子获得的726bp的克隆已在小麦属植物中得到鉴定，证实该转座子含有3个外显子(342、88、296)和2个内含子(750、1,237)。转座子的整个结构与已知的转座元件不同，则将该转座子命名为Revolver。Revolver的推导基因编码一种具有转录调节功能的蛋白质，具有DNA结合能力。小麦科植物基因组Southern杂交结果表明，Revolver在小麦近缘种黑麦、Dasypyrum以及小麦祖先种T.monoccum和Ae.基因组中含量丰富。方格罗萨。至少有一万份Revolver拷贝分布在小麦近缘的每条染色体上。然而，在面包小麦品种中，Revolver是相当罕见的。这些事实表明，自小麦的祖先进化以来，Revolver一直存在，然后在一些物种中扩增，而在面包小麦中消失。一类新的类转座子基因旋转器可用于野生小麦亲缘关系DNA标记的开发、小麦种质资源的开发和系统进化的评价