Physiological and Molecular Biological Analyses of seed Germination Ecology in Echinochloa crus-galli (L.) Beauv., a weed in flooded rice

稻田杂草稗草种子萌发生态的生理和分子生物学分析

• 批准号: 16580011
• 负责人: YAMASUE Yuji
• 金额: $ 2.3万
• 依托单位: Kyoto University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580011/
• 关键词: Echinocloa plants; Seed ecology; Anaerobic germination; Flooding-tolerance; Flooding-susceptibility; Alcohol fermentation; Pastuer effcet; ADH inhibitor; V-PPase; Echinochloa; 水田雑草; ヒメタイヌビエ; ヒエ属雑草; 種子発芽; 雑草の発芽生態

Flooding-tolerant and -susceptible varieties of Echinochloa crus-galli (L.) Beauv. were used to study (1) physiological and molecular analyses of the flooding tolerant seed germination and (2) metabolic regulation of seed respiration under flooding.1. Physiological and molecular analyses of flooding-tolerant seed germinationIn flooding-tolerant variety, seeds imbibed under N_2 could exhibited large I/N quotient and accelerated glycolysis and concomitantly produced large and equimolar quantities of CO_2 and ethanol, suggesting that the seeds operated the Pasteur effect and could germinate under anaerobic conditions. In contrast, seeds of the flooding-susceptible variety were capable of germinating only under aerobic conditions through the conventional aerobic respiration, but unable to anaerobically respire to germinate through the alcohol fermentation.2. Metabolic regulation of seed respiration under floodingIn a rice field where the soil is flooded, seeds of E.oryzicola anaerobically initially respire through alcohol fermentation to germinate and the seedlings switch the fermentation to the conventional aerobic respiration when they reach the water surface. This switching was found to be regulated by an endogenous ADH-inhibiting protein that is induced by oxygen.

对稗草（Echinochloa crus-galli（L.）博夫研究了（1）耐淹种子萌发的生理和分子分析;（2）淹水条件下种子呼吸的代谢调控.耐盐品种种子萌发的生理和分子生物学分析耐盐品种在N_2吸胀条件下能表现出较大的I/N商和加速糖酵解，并产生大量等摩尔量的CO_2和乙醇，表明种子具有巴斯德效应，能在厌氧条件下萌发。而感腐品种的种子只能在有氧条件下通过常规的有氧呼吸进行萌发，而不能通过厌氧呼吸进行酒精发酵萌发.淹水条件下种子呼吸的代谢调节在淹水的稻田中，稻瘟病菌种子最初通过酒精发酵进行厌氧呼吸，萌发后幼苗到达水面后将酒精发酵转换为常规的好氧呼吸。这种转换被发现是由内源性ADH抑制蛋白，是由氧诱导的调节。

项目成果

Differential Ability of Alcohol Fermentation between Seedsof Flooding-tolerant and -susceptible Varieties of Echinochloa crus-galli (L.) Beauv.

稗子耐水和敏感品种种子酒​​精发酵能力的差异。

• 期刊: Weed Biology and Management (査読中)
• 作者: K.Morimoto;Y.Yamasue
• 通讯作者: Y.Yamasue

Construction of a comparative RELP map of Echinochloa crus-galli using buffelgrass and other grass probes and quantitative trait loci analysis of flooding tolerance and other common traits in Echinpchloa crus-galli

利用水牛草等草类探针构建稗草比较RELP图谱，并对稗草耐水性和其他共同性状进行数量性状位点分析

• 发表时间: 2004
• 期刊: Theor.Appl.Genet 108
• 作者: Fukao;T.;Y.Yamasue;et al.
• 通讯作者: et al.

Crop-associated Weeds, In 「Weed Ecology and Management」(ed by S.Inderjit、分担執筆)

作物相关杂草，载于《杂草生态学与管理》（由 S. Inderjit 编辑，合著者）

• 发表时间: 2004
• 作者: Tominaga;T.;Y.Yamasue
• 通讯作者: Y.Yamasue