Plug seedling production by using simplified in vitro culture under the ambient condition,,sheet seedlings and microbe-static's by phosphate Refractoriness with Aluminum

在环境条件下使用简化的体外培养进行穴盘苗生产，片状苗和通过磷酸盐铝耐火性进行微生物抑制

• 批准号: 16580016
• 负责人: MIZUTA Youichi
• 金额: $ 2.37万
• 依托单位: KYOTO UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580016/
• 关键词: Available chlorine; Tissue culture; Sucrose fatty-acid ester; Plug seedling; Sterilization; Nisin; Revival; Damaged Bacteria; 育苗; 順化

The microorganism colony incidence rate in the plant tissue culture medium was suppressed to one or less every 10^9 liters by boiling after the sucrose fatty-acid ester was added to the medium made from tap water, and adding the 2mg/l of available chlorine.In addition, the medium with easy-deterioration organic compound such as the potato and the coconut water could be sterilized by adding nisin.It succeeded in making it to one microbial contamination rate for 1000 of the inoculation and explants with chlorine after the explants was immersed in the benomyl and penicillin, or protamine, under normal office ambient condition (about 500-300 colonies formed without these microbecide). As for the tissue culture with a solid medium, the culture operation will be able to be done by this method by having it at a practicable microbial contamination rate in any medium composition and vessel volume.The prospect is good from the media sterilized method only with chlorine and omitting even the boil of the medium.

在自来水配制的培养基中加入蔗糖脂肪酸酯后煮沸，并加入2 mg/l有效氯，可将植物组织培养基中的微生物菌落发生率抑制在每10^9升1个或1个以下。中，易。通过添加乳酸链球菌素，可以对马铃薯和椰子水等变质有机化合物进行杀菌，并成功地使其微生物污染率达到1，在正常办公室环境条件下，将外植体浸入苯菌灵和青霉素或鱼精蛋白中后，用氯接种1000 μ l外植体（在没有这些杀微生物剂的情况下形成约500-300个菌落）。至于用固体培养基进行的组织培养，在任何培养基组成和容器体积中，都能以可行的微生物污染率进行培养操作，从只用氯消毒甚至省略培养基煮沸的培养基消毒方法来看，前景是好的。

项目成果

塩素,ショ糖エステルおよびグリシンの添加が培地の煮沸殺菌の効果に及ぼす影響

添加氯、蔗糖酯和甘氨酸对培养基煮沸灭菌效果的影响

• 发表时间: 2005
• 期刊: 園芸学会雑誌 第74巻別冊2
• 作者: 水田洋一;東出奈穂子;矢澤 進
• 通讯作者: 矢澤 進

The influence of addition of the chlorine, sucrose ester and glycine on the effect of boilling sterilization of te media

氯、蔗糖酯、甘氨酸的添加对介质煮沸灭菌效果的影响

• 发表时间: 2005
• 期刊: Journal of the Japanese Sosiety for Horticultural Science 74(supplment2)
• 作者: Mizuta;Y.;N.Higashide;S.Yazawa
• 通讯作者: S.Yazawa

Many explants can inoculate under the ambient condition by enough wetting of the explants and vessels with a little, low pH and high density chlorine solution

许多外植体可以在环境条件下通过用少量、低pH值和高密度氯溶液充分润湿外植体和容器来接种

• 发表时间: 2005
• 期刊: Journal of the Japanese Society for Horticultural Science 74(supplment1)
• 作者: Mizuta;Y.;T.Hirota;S.Yazawa
• 通讯作者: S.Yazawa

Media with biotic compounds can be sterilized by sucrose ester addition and splitting addition of chlorine without autocraving

含有生物化合物的培养基可以通过添加蔗糖酯和分裂添加氯来灭菌，无需高压灭菌

• 发表时间: 2005
• 期刊: Journal of the Japanese Sosiety for Horticultural Science 74(supplment1)
• 作者: Mizuta;Y.;N.Higashide;S.Yazawa
• 通讯作者: S.Yazawa

塩素,ショ糖エステルおよびグリシンの添加が培地の煮沸殺菌の効果にぼす影響

添加氯、蔗糖酯和甘氨酸对培养基煮沸灭菌效果的影响

• 发表时间: 2005
• 期刊: 園芸学会雑誌 第74巻 別冊2
• 作者: 水田洋一;東出奈穂子;矢澤 進
• 通讯作者: 矢澤 進