Investigation of protein secreted from cultured cell line in insect

昆虫培养细胞系分泌蛋白质的研究

• 批准号: 16580037
• 负责人: HIRAOKA Tsuyoshi
• 金额: $ 2.37万
• 依托单位: Tokyo University of Agriculture and Technology
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580037/
• 关键词: insect culture cell; functional protein; secreted protein

Acetylcholinesterase was purified from conditioned medium of insect cell line NIAS-AeA1-2. SDS-PAGE was suggested that molecular weight was 67kDa, and gel-filtrated chromatography suggested this protein was dimmer in conditioned medium.Growth blocking peptide, an insect EGF type growth factor was detected by RT-PCR in SES-NaBr-4 and SES-NaBr-93 cell line.Trypsin inhibitor was detected in NIAS-AeA1-2, and SES-MaBr-4. I tried to purify this protein but in step of affinity column chromatography, this protein adsorbed tightly to trypsin. This protein still unclear about molecular weight and function.Dopa-decalboxilase was detected by RT-PCR in SES-NaBr-4 Cell line

从昆虫细胞系NIAS-AeA 1 -2的条件培养基中纯化乙酰胆碱酯酶。RT-PCR检测到SES-NaBr-4和SES-NaBr-93细胞中含有昆虫EGF型生长因子生长阻断肽，NIAS-AeA 1 -2和SES-MaBr-4细胞中含有胰蛋白酶抑制剂。我试图纯化该蛋白，但在亲和柱层析步骤中，该蛋白与胰蛋白酶紧密吸附。该蛋白的分子量和功能尚不清楚，RT-PCR检测到多巴脱羧酶在SES-NaBr-4细胞中的表达