Establishment of general procedure to synthesize polysialogangliosides and its application for neurobidogy.

聚唾液酸神经节苷脂合成通用工艺的建立及其在神经生物学中的应用。

• 批准号: 16580086
• 负责人: ISHIDA Hideharu
• 金额: $ 2.37万
• 依托单位: Gifu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580086/
• 关键词: ganglioside; sialic acid; ceramide; sphingo-glycolipid

Glycosphingolipids are characteristic membrane components of eukaryotic cells where they are distinguished from other glycosphingolipids by containing one or more sialic acid residues, and have been recognized play important roles in many biological processes, particularly in nervous system We have succeeded in the establishment of general procedunes to synthesize polysialogangliosides. The achievements are summarized in the following two parts.1.Efficient construction of polysialo-oligosaccharides.α(2-4)- and α(2-8)disialic acid seguences were obtained in high yields by employing 1,5-lactamized sialyl acceptors. The disaccharides obtained were utilized for the construction of sialo-oligosaccharides.2.The coupling of oligosaccharides and ceramides by intramolecular-aglycon derivery.Tethering of ceramide to glucose, reducing terminal of oligosaccharides, with succnic acid allowed the efficient coupling of oligosaccharides and ceramides. The glucosyl ceramide obtained was further glycosylated with sialo-oligosaccharides as building bloks to give the desired gangliosides.

鞘糖脂是真核细胞特有的膜成分，与其他鞘糖脂不同，它含有一个或多个唾液酸残基，在许多生物过程中，特别是在神经系统中发挥着重要作用。研究成果分为以下两部分。高效构建聚硅寡糖。α(2-4)-和α(2-8)双二酸序列采用1,5-内酰胺化的唾液酸受体高产得到。得到的二糖被用于构建唾液寡糖。寡糖与神经酰胺的分子内糖基传递偶联。将神经酰胺拴在葡萄糖上，用琥珀酸还原低聚糖末端，使低聚糖和神经酰胺有效偶联。得到的葡萄糖基神经酰胺与唾液寡糖作为构建块进一步糖基化，得到所需的神经节苷。

项目成果

Design and synthesis of a novel neo-glycolipid containing sialyl Lewis X determinant carried on the mucin GlcNAcb1-6GalNAca core structure

设计和合成一种新型新糖脂，含有粘蛋白GlcNAcb1-6GalNAca核心结构上的唾液酸Lewis X决定簇

• 发表时间: 2005
• 期刊: Tetrahedron : Asymmetry 16(7)
• 作者: Otsubo;N.
• 通讯作者: N.

Ando T. : Synthesis and enzymatic susceptibility of a series of novel GM2 analogs

Ando T.：一系列新型 GM2 类似物的合成和酶敏感性

• 发表时间: 2006
• 期刊: Glycoconjugate Journal 23
• 作者: Fuse;T.;Ando;H.;Imamura;A.;Sawada;N.;Ishida H.;kiso;M.
• 通讯作者: M.

Design and synthesis of a novel neo-glycolipid containing sialyl Lewsi X determinant carried on the mucin GlcNAcb1-6GalNAca core structure

设计和合成一种新型新糖脂，含有粘蛋白 GlcNAcb1-6GalNAca 核心结构上的唾液酸 Lewsi X 决定簇

• 发表时间: 2005
• 期刊: Tetrahedron : Asymmetry 16(7)
• 作者: Otsubo;N.
• 通讯作者: N.

糖類化学の最先端技術 The Recent Trends of Glycochemistry

糖化学的最新趋势

• 发表时间: 2005
• 作者: Yamaguchi;M.;Ishida;H.;Kanamori;A.;Kannagi;R.;Kiso;M.;石田 秀治;石田 秀治;安藤 弘宗;石田 秀治
• 通讯作者: 石田 秀治

Surface plasmon resonance study of carbohydrate-carbohydrate interaction between various gangliosides and Gg3-carring polystyrene

各种神经节苷脂与携带 Gg3 的聚苯乙烯之间碳水化合物-碳水化合物相互作用的表面等离子体共振研究

• 发表时间: 2004
• 期刊: Biomacromolecules 5
• 作者: Matsuura;K.;Oda;R.;Kitakouji;H.;Kiso;M.;Kitajima;K.;Kobayashi;K.
• 通讯作者: K.