Effects of conjugated linolenic acids on lipid metabolism and immune functions in rats.

共轭亚麻酸对大鼠脂质代谢和免疫功能的影响。

• 批准号: 16580107
• 负责人: KOBA Kazunori
• 金额: $ 2.37万
• 依托单位: Siebold University of Nagasaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580107/
• 关键词: conjugated linolenic acid; punicic acid; pomegranate seed oil; adipose tissue weight; fatty acid beta-oxidation; immunoglobulin; rats; 脂肪酸β酸化能; 脂肪酸合成能

Conjugated linolenic acid (CLN) is a fatty acid that has 18 carbons with three conjugated double bonds composed of multiple positional and geometric (cis(c) and trans(t)) isomers. Conjugated linoleic acid (CLA), the representative conjugated fatty acid has been shown to reduce body fat in rodents. We previously demonstrated that body fat reducing effect of dietary CLN is at least comparable or even stronger than that of CLA in rats. The CLN used in the study was prepared by alkaline isomerization, and was a mixture of conjugated diene and conjugated triene. It is not clear whether the effects of synthetic CLN are common to all CLN isomers or responsible to some specific isomers. In nature, some seed oil contains a particular type of CLN as a main fatty acid component. Bitter gourd, pomegranate, catalpa and pot marigold contain alpha-eleostearic acid (9c,11t,13t-18:3), punicic acid (9c,11t,13c-18:3), catalpic acid (9t,11t,13c-18:3), and calendic acid (8t,10t,12c-18:3), respectively. In this project, we examined whether the CLN in these seed oils reduced the adipose tissue weights in male SD rats. Linseed oil containing alpha-linolenic acid (LN) was used as a control fat. After 4 wk on diets containing 0.7% of these fats, dietary punicic acid (pomegranate oil) lowered, whereas the other CLNs did not lower the weight of perirenal adipose tissue as compared with LN. Also, punicic acid increased the activity of hepatic beta-oxidation in mitochondria. This effect was also partly observed in rats pre-fed with the high-fat (20% lard) diet for 4 wk. It was, therefore, suggested that punicic acid in pomegranate oil was at least one of the factors for reducing adipose tissue weight in rats. In this study, we also measured serum concentration of immunoglobulin (Ig) A, IgG, IgM and IgE. However, the dietary fat-dependent effect was not significant.

共轭亚麻酸(CLN)是一种含有18个碳原子和3个共轭双键的脂肪酸，由多个位置和几何异构体(顺(C)和反(T))组成。共轭亚油酸(CLA)是一种具有代表性的共轭脂肪酸，已被证明能降低啮齿动物的体脂。我们先前已经证明，饮食中的CLN的减脂作用至少与CLA相当，甚至更强。研究中使用的CLN是通过碱性异构化制备的，是共轭双烯和共轭三烯的混合物。目前尚不清楚合成的CLN的作用是对所有的CLN异构体都有影响，还是对某些特定的异构体有责任。在自然界中，一些种子油含有一种特殊类型的CLN作为主要脂肪酸成分。苦瓜、石榴、梓子和盆栽万寿菊分别含有α-十二烷酸(9c，11t，13t-18：3)、辛酸(9c，11t，13c-18：3)、没食子酸(9t，11t，13c-18：3)和决明酸(8t，10t，12c-18：3)。在这个项目中，我们研究了这些种子油中的CLN是否降低了雄性SD大鼠的脂肪组织重量。以含α-亚麻酸(LN)的亚麻籽油为对照脂肪。饲喂含0.7%这些脂肪的饲料4wk后，饲料中的肉酸(石榴油)降低，而其他CLN与LN相比，并没有降低肾周脂肪组织的重量。此外，番木酸还增加了线粒体中的肝脏β-氧化活性。在喂食高脂肪(20%猪油)4wk的大鼠中，也观察到了部分这种影响。因此，石榴油中的番茄酸至少是减轻大鼠脂肪组织重量的因素之一。同时测定血清免疫球蛋白(Ig)A、Ig G、Ig M和Ig E浓度。然而，膳食脂肪依赖效应并不显著。

项目成果

Dietary conjugated linolenic acid modifies body fat mass, and serum and liver lipid levels in rats.

膳食共轭亚麻酸可改变大鼠的体脂肪量以及血清和肝脏脂质水平。

• 发表时间: 2006
• 期刊: Dietary Fats and Risk of Chronic Disease ed. by Yung-Sheng HUANG, Teruyoshi YANAGITA, Howard R. KNAPP, AOCS press, 2006. 5.
• 作者: Kazunori KOBA;Asuka AKAHOSHI;Kazunari TANAKA;Kazuo MIYASHITTA;Toshio IWATA;Takeshi KAMEGAI;Takaya YAMAMOTO;Michihiro SUGANO
• 通讯作者: Michihiro SUGANO

Dietary Fats and Risk of Chronic Disease

膳食脂肪和慢性病的风险

• 发表时间: 2006
• 作者: T.Sugahara;K.Nakamoto;K.Hara;Kazunori KOBA
• 通讯作者: Kazunori KOBA