Transfer of genome information to reconstitute endangered birds

转移基因组信息以重建濒危鸟类

• 批准号: 16580227
• 负责人: ONO Tamao
• 金额: $ 2.18万
• 依托单位: Shinshu University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580227/
• 关键词: chicken; quail; blue-breasted quail; embryo genesis; primordial germ cells; chimera; PCR; antibody; in situハイブリダイゼーション

The aim of this project was the production and reserve of endangered, rare and valuable birds using the technique of germ cell transfer. We developed the protocol to obtain a large amount of PGCs from gonads of chicken and quail embryos using PGC specific monoclonal antibodies (anti-SSEA1, 2C9 and QCR1) and magnetism activated cell sorting (MACS). MACS treatment greatly increased the population ratio of avian PGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. Blastodermal and primordial germ cells were transferred to the recipient avian embryos. Treated chimeras were hatched and the donor-derived cells were tracked using species-specific DNA markers. Donor derived genome was observed using PCR and Immunohistochemistry in chimeric quail which introduced blastodermal cells of chicken and blue-breasted quail embryos.

该项目的目的是利用生殖细胞移植技术生产和保护濒危、珍稀和珍贵的鸟类。本研究采用PGC特异性单克隆抗体（抗SSEA1、2C9和QCR1）和磁激活细胞分选（MACS）技术，从鸡和鹌鹑胚胎性腺中大量获得PGC。MACS处理大大增加了鸟类PGCs在性腺细胞中的群体比例，从而改善了受体胚胎的性腺迁移。将胚盘细胞和原始生殖细胞转移到受体鸡胚中。孵化处理的嵌合体，并使用物种特异性DNA标记物追踪供体来源的细胞。利用PCR和免疫组化技术对鸡胚和蓝胸鹌鹑胚的囊胚细胞嵌合体进行了观察。

项目成果

Dynamic analysis of the developmental fate of cells in the center of the area pellucida of the blastoderm in chicken.

鸡胚层透明区中心细胞发育命运的动态分析。

• 发表时间: 2007
• 期刊: Journal of Poultry Science 44・1
• 作者: Yamamoto;Y.
• 通讯作者: Y.

Fertilization and blastoderm development of quail oocytes after intracytoplasmic injection of chicken sperm bearing a W chromosome.

胞浆内注射带有 W 染色体的鸡精子后鹌鹑卵母细胞的受精和胚盘发育。

• 发表时间: 2007
• 期刊: Poultry Science 86(5)
• 作者: Takagi S;Ono T;Shimada K.
• 通讯作者: Shimada K.

"Recessive Black" : A Plumage Color Mutant in Japanese Quail

“隐性黑色”：日本鹌鹑的羽毛颜色突变体

• 发表时间: 2005
• 期刊: The Journal of Poultry Science 42
• 作者: Fujiwara;Kagami et al.
• 通讯作者: Kagami et al.

Chicken polymorphism detection using RLGS and novel method, MONIC. (in Japanese).

使用 RLGS 和新方法 MONIC 进行鸡多态性检测。

• 发表时间: 2005
• 期刊: DNA Polymorphism (Toyo Shoten) Vol.13(Suppl.)
• 作者: Yamakawa;N.
• 通讯作者: N.

Enriched gonadal migration of donor-derived primordial germ cells by immunomagnetic cell sorting in birds.

通过免疫磁性细胞分选在鸟类中富集供体来源的原始生殖细胞的性腺迁移。

• 发表时间: 2004
• 期刊: Molecular Reproduction and Development 68・1
• 作者: Kim;J.N.
• 通讯作者: J.N.