Transfer of genome information to reconstitute endangered birds

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The aim of this project was the production and reserve of endangered, rare and valuable birds using the technique of germ cell transfer. We developed the protocol to obtain a large amount of PGCs from gonads of chicken and quail embryos using PGC specific monoclonal antibodies (anti-SSEA1, 2C9 and QCR1) and magnetism activated cell sorting (MACS). MACS treatment greatly increased the population ratio of avian PGCs in gonadal cells, resulting improved gonadal migration in recipient embryos. Blastodermal and primordial germ cells were transferred to the recipient avian embryos. Treated chimeras were hatched and the donor-derived cells were tracked using species-specific DNA markers. Donor derived genome was observed using PCR and Immunohistochemistry in chimeric quail which introduced blastodermal cells of chicken and blue-breasted quail embryos.