Molecular phylogenetic and evolutional studies of mitochondrial genome in the order Galliformes

鸡形目线粒体基因组的分子系统发育和进化研究

• 批准号: 16580231
• 负责人: NISHIBORI Masahide
• 金额: $ 2.3万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580231/
• 关键词: Galliformes; Molecular Phylogenetics; mitogenome; tribe; new world quail; ancestor of chicken; complete sequence of mitochondrial genome; morphological classification; ミトコンドリアDNA; 分子系統樹; 系統; ツカツクリ; ヤケイ; ウズラ

Domesticated fowls such as chicken, turkey, quail, and guineafowl are classically grouped in order Galliformes comprising 283 species. Galliformes consists of 5 families. The 283 species are grouped into 75 genera constituting 5 families. The current grouping has been performed on the basis of morphological features and genomic DNA-DNA hybridization. In the present study, in order to obtain a phylogenetic relationship of avian species grouped in Galliformes based on genome sequence, we have collected blood samples from 29 individuals belonging to 23 species (14 genera in 5 families in Galliformes), and determined the nucleotide sequence of their entire mitochondrial DNAs. Then, using the concatenated amino acid sequence deduced from 12 mitochondrial genes (excluding ND6 gene), phylogenetic analyses were performed by maximum parsimony, and Bayesian analysis methods. The analyses with the two different methods gave one and the same phylogenetic tree for the 23 species. The outline of the tree is as follows : (((((Phasianidae, Odontophoridae), Numididae), Cracidae), Megapodidae), Anseriformes). In the present study, two crucial findings for species classification have been revealed : one is that the species classically grouped in the tribes Phasianini (pheasant) and Perdicinin (partridge) were not grouped in corresponding monophyletic clusters. More precisely, species in Phasianini and species in Perdicinin formed monophyletic clusters. The other one is that Lagopus mutus and Melgaris Gallopavo are evolutionally much closer to genera Lophura than ever reported. These findings would contribute to species conservation and breeding of domesticated fowls.

鸡、火鸡、鹌鹑和珍珠鸡等家禽通常按鸡形目分类，共有 283 个物种。鸡形目由 5 个科组成。这283种物种分为5科75属。目前的分组是根据形态学特征和基因组 DNA-DNA 杂交进行的。在本研究中，为了根据基因组序列获得鸡形目鸟类的系统发育关系，我们采集了23个物种（鸡形目5科14属）的29只个体的血液样本，并测定了其整个线粒体DNA的核苷酸序列。然后，利用12个线粒体基因（不包括ND6基因）推导的氨基酸序列，通过最大简约法和贝叶斯分析方法进行系统发育分析。使用两种不同方法的分析给出了 23 个物种的同一个系统发育树。树的轮廓如下：((((雉科、齿雉科)、Numididae)、Cracidae)、Megapodidae)、Anseriformes)。在本研究中，揭示了物种分类的两个重要发现：其一是经典分组为雉鸡（Phasianini）和鹧鸪（Perdicinin）（鹧鸪）部落的物种并未分组到相应的单系群中。更准确地说，Phasianini 中的物种和 Perdicinin 中的物种形成单系簇。另一个是，Lagopus mutus 和 Melgaris Gallopavo 在进化上比以往报道的更接近 Lophura 属。这些发现将有助于物种保护和家禽繁殖。

项目成果

Karyotypic evolution in the Galliformes : An examination of the process of karyotypic evolution by comparison of themolecular cytogenetic findings with the molecular phylogeny.

鸡形目核型进化：通过分子细胞遗传学发现与分子系统发育的比较来检查核型进化过程。

• 发表时间: 2004
• 期刊: Cytogenet Genome Res 101
• 作者: Shibusawa;M.
• 通讯作者: M.

Molecular evidence for hybridization of species in the genus Gallus except for Gallus varius

• DOI: 10.1111/j.1365-2052.2005.01318.x
• 发表时间: 2005-10-01
• 期刊: ANIMAL GENETICS
• 影响因子: 2.4
• 作者: Nishibori, M;Shimogiri, T;Yasue, H
• 通讯作者: Yasue, H

Genetic variability and relationships of native Japanese chickens based on microsatellite DNA polymorphisms - Focusing on the natural monuments of Japan -.

基于微卫星 DNA 多态性的日本本土鸡的遗传变异和关系 - 关注日本的自然遗迹 -。

• 发表时间: 2006
• 期刊: Journal of Poultry Science 43
• 作者: Tahir;M.;Saleh;F.;Ohtsuka;A.;Hayashi;K.;Fumihiko Okumura;Masahide Nishibori;Masahide Nishibori;Bayed Abdel-Maksoud Osman
• 通讯作者: Bayed Abdel-Maksoud Osman

Plumage color and other morphologic characteristic variation of the native chicken in Cambodia

柬埔寨土鸡的羽毛颜色和其他形态特征变异

• 发表时间: 2006
• 期刊: Rep. Soc, Res. Native Livestock 23
• 作者: Tahir;M.;Saleh;F.;Ohtsuka;A.;Hayashi;K.;Fumihiko Okumura;Masahide Nishibori
• 通讯作者: Masahide Nishibori

Genetic variability and relationships of Japanese native chickens assessed by microsatellite DNA profiling - Focusing on the breeds established in Kochi Prefecture, Japan -

通过微卫星 DNA 分析评估日本本土鸡的遗传变异和关系 - 重点关注日本高知县建立的品种 -

• 发表时间: 2005
• 期刊: Asian Australia Journal of Animal Science 18
• 作者: Tahir;M.;Saleh;F.;Ohtsuka;A.;Hayashi;K.;Fumihiko Okumura;Masahide Nishibori;Masahide Nishibori;Bayed Abdel-Maksoud Osman;Masahide Nishibori;西堀正英;Masahide Nishibori;Yoshio Yamamoto;Sayed Abdel-Maksoud Osman
• 通讯作者: Sayed Abdel-Maksoud Osman