Create international standard SNP markers for chicken, and theiranalysis systems

创建国际标准的鸡SNP标记及其分析系统

• 批准号: 22580319
• 负责人: NISHIBORI Masahide
• 金额: $ 3万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2010
• 资助国家: 日本
• 起止时间: 2010 至 2012
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-22580319/
• 关键词: ニワトリ; SNP; マーカー; DigiTag2; 国際スタンダード; 96 組; 集団解析; 個体識別; 96組

Single nucleotide polymorphisms (SNPs) have been used to identify genes responsible for economic traits, including genetic diseases in domestic animals, and to examine genet ic diversity of populations. In this study, the aim is to c reate international standard SNP markers for chicken, and their analysis systems. I genotyped 239 chicken autosomal SNPs using DigiTag2 assay to understand the genetic structure of four species of Junglefowls, the Japanese native chicken breeds , and the relationship of these breeds. Phylogenetic analysis using the concatenated 74 autosomal SNP genotypes distinguished four Junglefowls, all chickens and formed clusters of chickens belonging to the respective breeds. Based on the allele frequency data, the estimated probability of identity (P) was calculated to be 2.70×10-20. Since the FAOSTAT database published in 2011 showed that the total number of chickens raised worldwide was approximately 1.99 × 1010, this SNP set would have the power to identify all chickens in the world. More importantly, this set would be particularly useful to monitor the genetic structure of populations for maintenance of the characteristics of the chicken breeds and junglefowls.

单核苷酸多态性（SNPs）已被用于鉴定负责经济性状的基因，包括家畜的遗传疾病，并检查种群的遗传多样性。本研究旨在建立国际标准的鸡SNP标记及其分析系统。我使用DigiTag 2分析法对239只鸡的常染色体SNPs进行了基因分型，以了解日本本土鸡种Junglefowls的遗传结构以及这些品种之间的关系。系统发育分析使用串联的74个常染色体SNP基因型区分四个Junglefowls，所有鸡，并形成集群的鸡属于各自的品种。根据等位基因频率数据，估计的同一性概率（P）计算为2.70×10-20。由于2011年公布的FAOSTAT数据库显示，全球饲养的鸡总数约为1.99 × 1010只，因此该SNP集将有能力识别世界上所有的鸡。更重要的是，这套将是特别有用的监测种群的遗传结构，以保持鸡品种和丛林鸡的特征。

项目成果

Production and price of indigenous naked neck and full feathered chicken reared under rural scavenging system in Bangladesh

孟加拉国农村拾荒系统饲养的本土裸颈鸡和全羽鸡的产量和价格

• 发表时间: 2012
• 期刊: Journal of Agricultural Extension and Rural Development
• 作者: Kozai;K.;Hojo;T.;Takahashi;M.;Acosta;TJ.;Nambo;Y.;Okuda;K;Mohammad F. Ahmed
• 通讯作者: Mohammad F. Ahmed

Islam. Production and price of indigenous naked neck and full feathered chicken reared under rural scavenging system in Bangladesh

伊斯兰教。

• 发表时间: 2012
• 期刊: Journal of Agricultural Extension and Rural Development
• 作者: Mohammad F. Ahmed;Masahide Nishibori,and Mohammed A
• 通讯作者: Masahide Nishibori,and Mohammed A

Japanese Silkie fowls are widely distributed in the phylogenetic tree derived from mitochondrial complete D-loop nucleotide sequences

日本乌骨鸡广泛分布于线粒体完整 D 环核苷酸序列的系统发育树中

• 发表时间: 2011
• 期刊: Journal of Poultry Science
• 影响因子: 1.5
• 作者: Jahan Rowshan;Miyuki Kumagae;Masahide Nishibori;Hiroshi Yasue and Yasuhiko Wada
• 通讯作者: Hiroshi Yasue and Yasuhiko Wada

Phylogenetic Analysis of Native Chicken from Bangladesh and Neighboring Asian Countries Based on Complete Sequence of Mitochondrial DNA D-loop Region

基于线粒体 DNA D 环区完整序列的孟加拉国及亚洲邻国土鸡的系统发育分析

• 发表时间: 2012
• 期刊: Journal of Poultry Science
• 影响因子: 1.5
• 作者: Mohammed A. Islam;and Masahide Nishibori
• 通讯作者: and Masahide Nishibori

Genetic relationships between Japanese native and commercial breeds using 70 chicken autosomal SNP genotypes by the DigiTag2 assay

• DOI: 10.1111/j.1365-2052.2011.02206.x
• 发表时间: 2012-02-01
• 期刊: ANIMAL GENETICS
• 影响因子: 2.4
• 作者: Shimogiri, T.;Nishida, N.;Yasue, H.
• 通讯作者: Yasue, H.