权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Characterization of ddY cataract mouse as a new animal model

ddY白内障小鼠作为新动物模型的表征

基本信息

批准号：
16580253
负责人：
OKADA Toshiya
金额：
$ 2.43万
依托单位：
Osaka Prefecture University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16580253/
关键词：
animal model cataract crystallin ddY mouse FGF lens protein linkage analysis TGF-β 白内障 EGF

项目摘要

The present study was designed to clarify the characters of inherited cataract of the ddY mice. For elucidation of the mode of inheritance, F1 and F2 hybrids between ddY cataract and normal ddY mice were analyzed. The cataract developed from 6 to 8 weeks old in the mutant but did not in the F1 hybrids. The ratio of affected to unaffected mice was 1:3 in the F2 hybrid. There was no sex difference in the incidence of the cataract. Therefore, the cataract is inherited by an autosomal recessive gene. The expressions of FGF and TGF-β were investigated by immunohistochemistry and the expression of lens protein by SDS-PAGE. The expressions of FGF and TGF-β were increased 3 to 6 weeks after birth. One of proteins that have decreased expression in cataract mice was β-crystallin B1. These findings revealed that the character is considered to be inherited by the autosomal recessive mode and that both the decrease in β-crystallin B1 and increase in FGF and TGF-β expressions are largely involved in the induction of cataract in this model. By linkage analysis, the cataract gene was localized in the region of 1.21 cM between D2mit 467, 515 (DNA microsatellite markers) and mitochondrial ribosome recycling factor (gene). These findings revealed that the ddY cataract mouse is new cataract model mouse and will be a good tool for genetic analysis and study of molecular biology of cataractogenesis.

本研究旨在阐明ddY小鼠遗传性白内障的特点。为了阐明遗传模式，对ddY白内障小鼠与正常小鼠的F1和F2杂交进行了分析。突变体的白内障在6 - 8周龄发育，而F1杂交体则没有。在F2杂交中，患病小鼠与未患病小鼠的比例为1:3。白内障的发生率无性别差异。因此，白内障是通过常染色体隐性基因遗传的。免疫组化法检测FGF和TGF-β的表达，SDS-PAGE法检测晶状体蛋白的表达。出生后3 ~ 6周FGF、TGF-β表达升高。在白内障小鼠中表达降低的蛋白之一是β-晶体蛋白B1。这些结果表明，该性状被认为是通过常染色体隐性模式遗传的，β-晶体蛋白B1的减少和FGF和TGF-β表达的增加在很大程度上参与了该模型中白内障的诱导。通过连锁分析，白内障基因定位在D2mit 467,515 （DNA微卫星标记）和线粒体核糖体再循环因子（基因）之间的1.21 cM区域。这些结果表明，ddY白内障小鼠是一种新的白内障模型小鼠，将为白内障发生的遗传分析和分子生物学研究提供良好的工具。