Characterization of CF-1 cataract mouse as a new animal model

CF-1白内障小鼠作为新动物模型的表征

• 批准号: 18500329
• 负责人: OKADA Toshiya
• 金额: $ 2.57万
• 依托单位: Osaka Prefecture University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18500329/
• 关键词: animal model; cataract; CF#1; b cac mouse; BALB; c mouse; linkage analysis; MSM; Ms mouse; modifier aene

The present study was designed to clarify the characters of inherited cataract of the CF#1 mice (CF#1/b cac). For elucidation of the mode of inheritance, Fl and F2 hybrids between CF#1/b cac and BALB/c mice were analyzed. The cataract developed between days 14 and 22 after birth in the mutant but did not in the Fl hybrids. The ratio of affected to unaffected mice was 1:3 in the F2 hybrid. There was no sex difference in the incidence of the cataract. Therefore, the cataract is inherited by an autosomal recessive gene. Two phenotypes (pin-head type and diffused type) were noted in the cataract animals from the F2 progenies between CF#1/b cac and BALB/c mice. The pin-head type developed between days 16 and 34 after birth and the diffused type between days 28 and 100. These findings reveal that the character of CF#1/b cac is considered to be inherited by the autosomal recessive mode and that BALB/c mice have modifier genes to the development of the cataract in CF#1/b cac. By linkage analysis with MSM/Ms mice, the cataract gene was localized in the region of 0.8 cM between D16mit92 (DNA microsatellite markers) and D16mit185. By the analysis with BALB/c mice, the modifier gene was localized in the region between D9Mit281 and D9Mit19. These findings revealed that the CF#1 cataract mouse is new cataract model mouse and will be a good tool for genetic analysis and study of molecular biology of cataractogenesis.

本研究旨在阐明CF#1小鼠（CF#1/b cac）遗传性白内障的特点。为了阐明遗传模式，我们分析了CF#1/b cac和BALB/c小鼠之间的Fl和F2杂交。在突变体中，白内障在出生后14天至22天发生，而在Fl杂交种中则没有。在F2杂交中，患病小鼠与未患病小鼠的比例为1:3。白内障的发生率无性别差异。因此，白内障是通过常染色体隐性基因遗传的。CF#1/b cac和BALB/c小鼠F2代白内障动物出现针尖型和弥漫性两种表型。针尖型在出生后16 ~ 34天发育，扩散型在出生后28 ~ 100天发育。这些结果表明，CF#1/b cac的特征被认为是通过常染色体隐性模式遗传的，并且BALB/c小鼠在CF#1/b cac的白内障发展中具有修饰基因。通过与MSM/Ms小鼠的连锁分析，白内障基因定位在D16mit92 （DNA微卫星标记）和D16mit185之间0.8 cM的区域。通过BALB/c小鼠的分析，修饰基因定位在D9Mit281和D9Mit19之间的区域。这些结果表明，CF#1白内障小鼠是一种新的白内障模型小鼠，将为白内障发生的遗传分析和分子生物学研究提供良好的工具。

项目成果

Study on the cataract found in CF#1 mice (CF#1/b cac Ta): morphological and hereditary characteristics

CF中发现白内障的研究

• 发表时间: 2008
• 期刊: Kansai Journal of Laboratory animals 29(in press)
• 作者: Okada;Toshiya;et. al.
• 通讯作者: et. al.

CF#1系由来遺伝性白内障マウスに関する研究 : 遺伝様式の解明

CF1品系遗传性白内障小鼠的研究：阐明遗传模式

• 发表时间: 2007
• 期刊: 第47回日本先天異常学会学術集会講演要旨集 (印刷中)
• 作者: Okada;Toshiya;et. al.;岡田利也
• 通讯作者: 岡田利也

CF#1系由来遺伝性白内障マウス(CF#1/b cac Ta)の形態学的特性ならびに遺伝様式に関する研究

CF1品系（CF1/b cac Ta）遗传性白内障小鼠形态特征及遗传模式研究

• 发表时间: 2008
• 期刊: 関西実験動物研究会報 29
• 作者: Hiroml Uramoto;Nobuyuki Takahashi and Yasunobu Okada;Hiromi Uramoto;Hiromi Uramoto;Hiromi Uramoto;Hiromi Uramoto;岡田 利也
• 通讯作者: 岡田 利也

CF#1系由来遺伝性白内障マウス(CF#1/bcac Ta)の形態学的特性ならびに遺伝様式に関する研究

CF1品系（CF1/bcac Ta）遗传性白内障小鼠形态特征及遗传模式研究

• 发表时间: 2008
• 期刊: 関西実験動物研究会報 29(印刷中)
• 作者: Okada;Toshiya;et. al.;岡田 利也
• 通讯作者: 岡田 利也

Hereditary characteristics of inherited cataract found in CF#1 mice

CF中发现的遗传性白内障的遗传特征

• 发表时间: 2007
• 期刊: Congenital Anomalies 47
• 作者: Okada;T.
• 通讯作者: T.