Characterization of CF-1 cataract mouse as a new animal model

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The present study was designed to clarify the characters of inherited cataract of the CF#1 mice (CF#1/b cac). For elucidation of the mode of inheritance, Fl and F2 hybrids between CF#1/b cac and BALB/c mice were analyzed. The cataract developed between days 14 and 22 after birth in the mutant but did not in the Fl hybrids. The ratio of affected to unaffected mice was 1:3 in the F2 hybrid. There was no sex difference in the incidence of the cataract. Therefore, the cataract is inherited by an autosomal recessive gene. Two phenotypes (pin-head type and diffused type) were noted in the cataract animals from the F2 progenies between CF#1/b cac and BALB/c mice. The pin-head type developed between days 16 and 34 after birth and the diffused type between days 28 and 100. These findings reveal that the character of CF#1/b cac is considered to be inherited by the autosomal recessive mode and that BALB/c mice have modifier genes to the development of the cataract in CF#1/b cac. By linkage analysis with MSM/Ms mice, the cataract gene was localized in the region of 0.8 cM between D16mit92 (DNA microsatellite markers) and D16mit185. By the analysis with BALB/c mice, the modifier gene was localized in the region between D9Mit281 and D9Mit19. These findings revealed that the CF#1 cataract mouse is new cataract model mouse and will be a good tool for genetic analysis and study of molecular biology of cataractogenesis.