Does the bovine lactoferrin improve chronic inflammation in the immuno-suppressive condition induced by FIV infection?

点击翻译按钮获取中文摘要

1)The effects of orally-administered bovine lactoferrin (bLF) on lymphocyte functions in FIV-infected catsThe effects of bLF on ConA-induced proliferation of peripheral lymphocyte in healthy and FIV-infected cats were investigated. Orally-administered bLF showed the inhibition of lymphocyte proliferation both in healthy and FIV- infected cats. Co-incubation of bLF and peripheral lymphocytes also showed the inhibition of lymphocyte proliferation both in healthy and FIV-infected cats.2)The effects of bLF and ConA on cytokine mRNA expression in feline peripheral lymphocyteAfter the co-incubation of ConA and peripheral lymphocyte, mRNA expression of IFN-γ,IL-1β,TNF-α and IL-12p40 was investigated by RT-PCR method. The strong mRNA-expression of IFN-γ was first detected at 4hr after the addition of ConA, however the addition of ConA did not bring the mRNA-expression of other cytokines such as IL-1(3,TNF-α and IL-12p40. After that, the mRNA-expression of IFN-γ was investigated by quantitative … More analysis with real time PCR in order to confirm the result of RT-PCR. We established the method of real time PCR for feline IFN-γ. After the stimulation of ConA, the strong mRNA-expression of IFN-γ was observed in peripheral lymphocyte both of healthy and FIV-infected cats, using real time PCR method. Before ConA stimulation and 10, 20 and 40 min after ConA stimulation, bLF was added to peripheral lymphocyte and ConA-induced mRNA- expression of IFN-γ was determined by real time PCR. Bovine LF inhibited the ConA-induced mRNA-expression of IFN-γ in felime lymphocyte. The inhibition of IFN-γ-mRNA-expression was observed not only when lymphocyte was treated with bLF 30 min before but also 10,20 or 40 min after ConA stimulation.3)The effects of Con A stimulation on the lymphocyte signaling pathways of IFN-γ-mRNA-expressionThe relation of ConA stimulation and signaling pathways of lymphocyte was investigated. Our results suggested that PTK-and ERK-dependent signaling pathways are involved in the ConA-induced expression of IFN-γ-mRNA. Therefore, it is likely that bLF inhibits IFN-γ production by interfering with this signaling pathway.Judging from these results, the beneficial effects of bLF in vivo may be explained by modulatory ability of bLF against feline lymphocyte. These modulatory actions of bLF seemed to be involved in anti-inflammatory effects on the cats with chronic inflammation. Less