CLINICAL APPLICATION OF URINARY NAG INDEX AND SDS-PAGE ANALYSIS OF URINARY PROTEIN IN FELINE AND CANINE URINARY DISORDER

尿NAG指数和尿蛋白SDS-PAGE分析在猫科和犬类泌尿系统疾病中的临床应用

• 批准号: 12833003
• 负责人: SATO Reeko
• 金额: $ 2.3万
• 依托单位: IWATE UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2000
• 资助国家: 日本
• 起止时间: 2000 至 2001
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-12833003/
• 关键词: URINARY NAG INDEX; SDS-PAGE; URINARY PROTEIN; NEPHROSIS MODEL; COWS; CATS; DOGS; NAG(NAGase); 尿; 尿蛋白; JDS-PAGE; 腎臓病; アイソザイム; 腎臓

This study was undertaken to determine the clinical usefulness of measurement of urinary NAG index and SDS-PAGE analysis of urinary protein as a marker for urinary disorder in animals. The results were as follows :(1) Urinary NAG index elevated from the early stage of nephrosis model experimentally-induced by potassium dichromate or sulfonamide in cattle and cat. Isoenzyme B of urinary NAG was increased after the administration of renal toxic drugs. (2) Urinary NAG index of the cases with chronic or acute renal failure was higher than that of healthy animals. On the other hand, NAG index of the cases with feline lower urinary tract disease or lower urinary tract infection was in the normal range. The dogs with lower urinary tract infection accompanied by pyelonephritis showed elevated values of NAG index. The elevation of NAG index indicated the extension of infection and inflammation to the pelvis. Therefore, measurement of urinary NAG index seemed to be useful as a marker of pyelonephritis. In secondary renal failure such as diabetes mellitus and pyometra, observation of urinary NAG index was also useful as a marker of renal injury. (3) In urine of chronic renal failure, low molecular proteins such as retinol-binding protein were detected by SDS-PAGE assay. In the assay of SDS-PAGE, serum protein pattern was observed in urine of lower urinary tract infection. SDS-PAGE assay of urinary protein was useful to distinguish the parenchymal lesions between kidneys and lower urinary tract. SDS-PAGE assay of urinary protein was more effective when it was done with the measurement of urinary NAG index. In conclusion, the clinical usefulness of measurement of urinary NAG and SDS-PAGE assay of urinary protein as the markers for renal diseases in animals were attested from the results obtained in this study.

本研究旨在确定尿NAG指数测定和尿蛋白SDS-PAGE分析作为动物泌尿系统疾病标志物的临床实用性。结果表明：（1）重铬酸钾或磺胺类药物诱发的牛、猫肾病模型，早期尿NAG指数升高，肾毒性药物给药后尿NAG同工酶B升高。(2)慢性和急性肾功能衰竭患者尿NAG指数均高于健康动物。而猫下尿路疾病或下尿路感染患者的NAG指数在正常范围内。下尿路感染伴肾盂肾炎犬NAG指数升高。NAG指数升高提示感染和炎症向盆腔的扩展。因此，尿NAG指数的测定似乎是有用的肾盂肾炎的标志物。对于糖尿病、子宫积脓等继发性肾功能衰竭患者，尿NAG指数的测定也可作为肾损伤的一个指标。(3)用SDS-PAGE法检测慢性肾功能衰竭患者尿中视黄醇结合蛋白等小分子蛋白。在SDS-PAGE分析中，观察了下尿路感染患者尿液中的血清蛋白谱。尿蛋白SDS-PAGE分析有助于区分肾脏和下尿路的实质病变。SDS-PAGE法测定尿蛋白与尿NAG指数联合测定更为有效。总之，本研究结果证实了尿NAG测定和尿蛋白SDS-PAGE测定作为动物肾脏疾病标志物的临床实用性。

项目成果

Reeko Sato: "Function and Clinical appiication of Factoferrin in small animals"Small Animal Clinic. 119. 12-21 (2000)

Reeko Sato：“Factoferrin 在小动物中的功能和临床应用”小动物诊所。

Jun Yasuda et. al.: "Idiopathic thrombocgtopenia in Japanease Black cattle"J. Vet. Med. Sci. 64. 87-89 (2002)

安田淳等。

Jun Yasuda et al.: "Idiopathic thrombocytopenia in Japanese Black cattle"J. Vet. Med. Sci.. 64. 87-89 (2002)

Jun Yasuda 等：“日本黑牛特发性血小板减少症”J.

Jun Sato et. al.: "High levels of serum alkaline phosphatase activities and its isoengymes in canine cases of pygmetra"J. Vet. Med. Assoc. (in press). (2002)

佐藤淳等。

Reeko Sato: "Function and clinical application of lactoferrin in small animals"Small Animal Clinic. 119. 12-21 (2000)

Reeko Sato：《乳铁蛋白在小动物中的功能及临床应用》小动物诊所。