A BASIC STUDY ON INDUCTION OF APOPTOSIS IN COWS WITH FATTY LIVER

奶牛脂肪肝是围产期疾病的主要发病机制之一。这种损害是由分娩前后的能量不平衡引起的。阐明其发病机制是控制围产期疾病的必要条件。在这项研究中，细胞凋亡的奶牛脂肪肝的发展进行了评估，通过使用4天的禁食模型先前报道（实验1）。此外，我们评估了过渡期内奶牛肝脏中细胞凋亡的诱导（实验2）。实验11.禁食4天后，肝脏甘油三酯（TG）含量增加，出现脂肪肝; 2.彗星试验观察到脂肪肝形成过程中肝细胞核碎裂增多; 3.免疫组化分析显示，脂肪肝形成过程中，肝细胞核碎裂增多;在脂肪肝的发生发展过程中，可观察到凋亡诱导相关抗体的阳性细胞。4.超微形态学分析，实验21.第3天和第21天肝脏TG含量比产犊前增加一倍以上; 2.第3天至第21天肝细胞核碎片增多。此外，使用上述免疫组织学分析观察阳性细胞。3.彗星试验中肝脏TG含量与尾矩呈显著正相关（r=0.68），在实验性脂肪肝和正常产犊后，脂肪肝奶牛肝脏TG含量均呈显著正相关（r=0.68）。这种现象被认为是对生命体的控制。我们计划研究脂肪肝中细胞凋亡的触发因素。

The fatty liver in dairy cattle is known as a primary pathogenesis of periparturient diseases. This lesion is induced by energy imbalance around parturition. The elucidation of the pathogenesis is indispensable to control periparturient diseases. In this study, apoptosis in the development of fatty liver of cows was evaluated by use of 4-days-fasting model reported previously (Exp.1). Further we assessed the induction of apoptosis in the livers of cows through transition period (Exp.2). Obtained data are as the followings.Exp.11.By fasting for 4 days, liver triglyceride (TG) content was increased and fatty liver was admitted.2.The increase of nuclear fragmentation in hepatic cells was observed in the development of fatty liver by use of comet assay.3.In immunohistological analysis, the positive cells to antibodies related to apoptosis induction were observe in the development of fatty liver.4.In ultramorphological analysis, apoptotic body was verified.Exp.21.The liver TG contents at day 3 and 21 were more than twice as much as that before calving.2.The nuclear fragmentation in hepatic cells was increased from day 3 to day 21. Further, the positive cells were observed using above-mentioned immunohistological analysis. Apoptotic body was also verified.3.The relationship between liver TG content and tail moment in comet assay was significantly positive (r=0.68).The induction of apoptosis in cows with fatty liver was verified in the experimental fatty liver and after usual calving. The phenomenon was considered the control of a living body. We are planning to study about the triggers of apoptosis in the fatty liver.