Development of simultaneous measurement of micro/macro-molecular kinetics in a living cell by laser speckle microscopy

通过激光散斑显微镜同时测量活细胞中的微观/宏观分子动力学的发展

• 批准号: 16590028
• 负责人: HIRAKAWA Yasuyuki
• 金额: $ 2.24万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590028/
• 关键词: Cell Observation; Laser Speckle; Cellular Activity; Fluorescence Observation

A laser speckle microscopy imaging system for observation of living cells was developed. This system consists of an optical microscope, a laser source, and a video camera. The system has an advantage in that cellular conditions could be instantaneously determined by observing speckle movements without any special preparation such as staining and genetic manipulation. In the experiments, the speckle fluctuations of living and fixed HeLa cells were compared with the change in the temperature of the culture medium around the cells, and their video images were quantitatively analyzed. Laser speckle fluctuations on these cells were also analyzed by using frequency spectra of speckle fluctuations. In this frequency analysis, particular peak around a few Hz was observed in a living cell. It was also found in this study that by using a femto second laser illumination, simultaneous observation of auto-fluorescence from bio-molecules and laser speckle in a living cell could be possible in this laser speckle microscopic system. By applying this system, it is possible to estimate not only cellular activity but also real-time movements of intercellular materials including the cytoplasm without requiring complicated labeling processes.

研制了一种用于活体细胞观察的激光散斑显微成像系统。该系统由光学显微镜、激光光源和摄像机组成。该系统的优点在于，细胞条件可以通过观察散斑运动来即时确定，而无需任何特殊的准备，如染色和遗传操作。在实验中，将活的和固定的HeLa细胞的斑点波动与细胞周围培养基的温度变化进行了比较，并对其视频图像进行了定量分析。利用散斑起伏的频谱分析了这些细胞上的激光散斑起伏。在该频率分析中，在活细胞中观察到几Hz附近的特定峰。在这项研究中还发现，通过使用飞秒激光照明，可以在这种激光散斑显微系统中同时观察生物分子的自发荧光和活细胞中的激光散斑。通过应用该系统，不仅可以估计细胞活性，而且可以估计包括细胞质在内的细胞间物质的实时运动，而不需要复杂的标记过程。

项目成果

Laser Speckle Microscope for Instanteneous Determination of Condition of Single Living Cell

用于即时测定单个活细胞状况的激光散斑显微镜

• 发表时间: 2005
• 期刊: Japanese Journal of Applied Physics 44・2
• 作者: H.Muratake;M.Natsume;H.Nakai;Y.Hirakawa
• 通讯作者: Y.Hirakawa

Frequency Analysis of Single Living Cell Activity by Laser Speckle Microscope

激光散斑显微镜对单个活细胞活动的频率分析

• 发表时间: 2005
• 期刊: Technical Digest of Conference on Lasers and Electro-Optics/Quantum Electronics and Laser Science and Photonic Applications, Systems and Technologies 2005
• 作者: H.Takahashi;T.Fukuda;H.Mitsuzuka;R.Namme;H.Miyamoto;Y.Ohkura;S.Ikegami^*;Y.Hirakawa
• 通讯作者: Y.Hirakawa

Recent Developments in Lasers and their Applicaiotions (Ch.7) "Recent Progress of Laser Microscopes"

激光及其应用的最新进展（第七章）“激光显微镜的最新进展”

• 发表时间: 2006
• 作者: H.Muratake;M.Natsume;Yasuyuki Hirakawa (N.Vasa eds.)
• 通讯作者: Yasuyuki Hirakawa (N.Vasa eds.)

Recent Progress of Laser Microscopes in Recent Developments in Lasers and their Applicaiotions (Chapter 7)

激光显微镜的最新进展及其应用（第七章）

• 发表时间: 2006
• 作者: Hanzawa;Y.et al.;Yasuyuki Hirakawa (N. Vasa eds.)
• 通讯作者: Yasuyuki Hirakawa (N. Vasa eds.)

Recent Progress of Laser Microscopes

激光显微镜的最新进展

• 发表时间: 2006
• 期刊: Recent Developments in Lasers and their Applications (N.Vasa eds.)(Research Signpost)
• 作者: Goda;H.;S.K.Nair et al.;Koyo Nishida;Koyo Nishida;Koyo Nishida;Koyo Nishida;Koyo Nishida;Koyo Nishida;Koyo Nishida;Koyo Nishida;Koyo Nishida;Koyo Nishida;Koyo Nishida;Koyo Nishida;Y.Hirakawa
• 通讯作者: Y.Hirakawa