Comparative functional genomics of fungal morphogenesis

真菌形态发生的比较功能基因组学

• 批准号: 47120453
• 负责人: Privatdozentin Dr. Minou Nowrousian
• 金额: --
• 依托单位: Lehrstuhl für Molekulare und Zelluläre Botanik
• 依托单位国家: 德国
• 项目类别: Research Grants
• 财政年份: 2007
• 资助国家: 德国
• 起止时间: 2006-12-31 至 2010-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/47120453?language=en
• 关键词: Comparative; functional; genomics; fungal; morphogenesis

This project aims at identifying evolutionary conserved patterns of gene expression during fungal morphogenesis using a systems biology approach. Large scale analyses of genes that are differentially expressed during fruiting body development will be performed for the ascomycetes Neurospora crassa and Pyronema confluens. The expression results will be compared to available data from the ascomycete Fusarium graminearum to identify genes that are differentially expressed during fruiting body development in all three species. The fruiting bodies of the three species are homologous structures; however, N. crassa and F. graminearum (Sordariomycetes) form an advanced type of fruiting body whereas P. confluens is a member of the basal group of Pezizomycetes and forms a simpler fruiting body. Genes that display conserved expression patterns in all three species should define a core group of factors that might be involved in basic pattern formation of the fungal fruiting body. Suitable candidate genes will be analyzed further using N. crassa knockout mutants to identify novel developmental genes.

本项目旨在利用系统生物学方法，鉴定真菌形态发生过程中基因表达的进化保守模式。将对子囊菌纲粗糙脉孢菌和融合火丝菌在子实体发育过程中差异表达的基因进行大规模分析。将表达结果与来自禾谷镰刀菌的可用数据进行比较，以鉴定在所有三个物种的子实体发育期间差异表达的基因。三种植物的子实体均为同源结构，而N. crassa和F.禾谷孢霉（Sordariomycetes）形成高级类型的子实体，而P.confluens是盘菌纲的基础组的成员，并形成简单的子实体。在所有三个物种中显示保守表达模式的基因应该定义一组核心因子，这些因子可能参与真菌子实体的基本模式形成。合适的候选基因将使用N. crassa基因敲除突变体，以确定新的发育基因。