MafA differentiates rat intestinal cells into insulin-producing cells.

MafA 将大鼠肠细胞分化为产生胰岛素的细胞。

• 批准号: 16590143
• 负责人: NAKAMURA Takaaki
• 金额: $ 1.66万
• 依托单位: Shiga University of Medical Science
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590143/
• 关键词: transcription factor; Mafa; rat intestine; diabetes mellitus; Pdx1; insulin; gene therapy; Pdx1; Isl-1; アデノウイルス; 転写調節因子

Recent studies have suggested that basic leucine zipper transcription factor MafA has a crucial role in pancreatic □-cell-specific insulin gene transcription. Thus, we investigated whether MafA overexpression in the intestine induces insulin production in small-intestinal epithelial cells in vivo. Recombinant adenovirus containing MafA gene(Ad-MafA) was prepared and administered orally to streptozocin-treated diabetic rats. Insulin gene expression was observed in the intestine by RT-PCR analysis, and then insulin protein was detected by immunohistochemical analysis after Ad-MafA administration. Furthermore, MafA overexpression in the intestine increased plasma insulin levels and ameliorated hyperglycemia. These results indicate that MafA overexpression in the intestine induces intestinal epithelial cells newly to produce and release insulin.

最近的研究表明亮氨酸拉链转录因子MafA在胰腺细胞特异性胰岛素基因转录中起着至关重要的作用。因此，我们在体内研究了小肠中MafA的过表达是否会诱导小肠上皮细胞产生胰岛素。制备了含MafA基因的重组腺病毒（Ad-MafA），并口服给药于链脲佐菌素治疗的糖尿病大鼠。采用RT-PCR法观察胰岛素基因在肠道内的表达，并在给药后采用免疫组化法检测胰岛素蛋白水平。此外，肠道中MafA的过表达增加了血浆胰岛素水平并改善了高血糖。这些结果表明，肠内MafA过表达诱导肠上皮细胞产生和释放新的胰岛素。

项目成果

Diet high in lipid hydroperoxide by vitamin E deficiency induces insulin resistance and impaired insulin secretion in normal rats.

由于维生素 E 缺乏而导致的高脂质过氧化氢饮食会导致正常大鼠的胰岛素抵抗和胰岛素分泌受损。

• 发表时间: 2005
• 期刊: Diabetes Research and Clinical i 67
• 作者: K.Tsujinaka;Takaaki Nakamura
• 通讯作者: Takaaki Nakamura

Sumoylation of Pdx1 is associated with its nuclear localization and insulin gene activation

• DOI: 10.1152/ajpendo.00390.2002
• 发表时间: 2003-04-01
• 期刊: AMERICAN JOURNAL OF PHYSIOLOGY-ENDOCRINOLOGY AND METABOLISM
• 影响因子: 5.1
• 作者: Kishi, A;Nakamura, T;Kashiwagi, A
• 通讯作者: Kashiwagi, A

Regulation of ATP-sensitive potassium channel subunit Kir6.2 expression in rat intestinal insulin-producing progenitor cells

• DOI: 10.1074/jbc.m410759200
• 发表时间: 2005-01-21
• 期刊: JOURNAL OF BIOLOGICAL CHEMISTRY
• 影响因子: 4.8
• 作者: Hashimoto, T;Nakamura, T;Kashiwagi, A
• 通讯作者: Kashiwagi, A

MafA differentiates rat intestinal cells into insulin-producing cells

• DOI: 10.1016/j.bbrc.2006.08.032
• 发表时间: 2006-10-13
• 期刊: BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
• 影响因子: 3.1
• 作者: Nomura, Satoshi;Nakamura, Takaaki;Kashiwagi, Atsunori
• 通讯作者: Kashiwagi, Atsunori