Analysis and Development of Anti-inflammatory peptide derived from Annexin 1

膜联蛋白1抗炎肽的分析与开发

• 批准号: 16590252
• 负责人: SATO Eisuke
• 金额: $ 2.3万
• 依托单位: Osaka City University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590252/
• 关键词: Annexin 1; Inflammation; peptide; Transgenic mice; neutrophils; superoxide; endotoxin; 腹腔; プロスタクランジン

Annexin 1 is a member of annexin superfamily proteins that bind to membrane phospholipids in a calcium-dependent manner. The N-terminal domains of these proteins have unique properties to exhibit their specific functions in various cells. We previously reported that annexin 1 is particularly abundant in neutrophils where it represents up to 3% of the cytosolic proteins. Because the majority of annexin 1 found in inflammatory tissues is associated with neutrophils, this protein might play important roles in the modulation of inflammatory processes. To analyze the physiological roles of annexin 1, we established human annexin1-transgenic mice. The transgenic mice had normal. phenotype and reproduction. Superoxide generation from neutrophils of annexin 1-transgenic mice slightly decreased. The population of neutrophils in blood also decreased. Annexin 1-transgenic mice dramatically blocks lipopolysaccharide (LPS)-stimulated neutrophil migration. Furthermore, we show that neutrophils recruitment induced by casein is suppressed also in annexin 1-transgenic mice. Based on such observations, physiological roles of annexin 1 in vivo will be discussed.

膜联蛋白1是膜联蛋白超家族蛋白质的成员，其以钙依赖性方式与膜磷脂结合。这些蛋白质的N端结构域具有独特的性质，在各种细胞中表现出其特定的功能。我们以前报道，膜联蛋白1是特别丰富的中性粒细胞，它代表了高达3%的胞浆蛋白。由于在炎症组织中发现的大多数膜联蛋白1与中性粒细胞相关，因此该蛋白可能在炎症过程的调节中发挥重要作用。为了分析膜联蛋白1的生理作用，我们建立了人膜联蛋白1转基因小鼠。转基因小鼠正常。表型和繁殖。膜联蛋白1转基因小鼠中性粒细胞产生的超氧化物略有减少。血液中的中性粒细胞数量也减少。膜联蛋白1转基因小鼠显著阻断脂多糖（LPS）刺激的中性粒细胞迁移。此外，我们发现，酪蛋白诱导的中性粒细胞募集也被抑制在膜联蛋白1转基因小鼠。基于这些观察，将讨论膜联蛋白1在体内的生理作用。

项目成果

L-carnitine inhibits hypoglycemia-induced brain damage in the rat

• DOI: 10.1016/j.brainres.2005.06.062
• 发表时间: 2005-08-16
• 期刊: BRAIN RESEARCH
• 影响因子: 2.9
• 作者: Hino, K;Nishikawa, M;Inoue, M
• 通讯作者: Inoue, M

Enhancing effect of an endocrine disruptor para-nonylphenol on the generation of reactive oxygen species in human blood cells

内分泌干​​扰物对壬基酚对人血细胞中活性氧产生的增强作用

• 发表时间: 2004
• 期刊: Environ Health Persp. 112・5
• 作者: Y.Okai;EF.Sato;K.Higashi-Okai;M.Inoue
• 通讯作者: M.Inoue

ストレスの辞典

重音词典

• 发表时间: 2005
• 作者: Numabe H;Ikeuchi T;Kajii T;Kusakawa I;Sato I;Kosugi S;佐藤英介
• 通讯作者: 佐藤英介

Effect of 17β-estradiol on immunosuppression induced by ultraviolet B irradiation

• DOI: 10.1007/s00403-003-0437-0
• 发表时间: 2004-02-01
• 期刊: ARCHIVES OF DERMATOLOGICAL RESEARCH
• 影响因子: 3
• 作者: Hiramoto, K;Tanaka, H;Inoue, M
• 通讯作者: Inoue, M

Effect of endocrine disruptor para-nonylphenol on the cell growth and oxygen radical generation in Escherichia coli mutant cells deficient in catalase and superoxide dismutase

• DOI: 10.1016/j.freeradbiomed.2004.07.001
• 发表时间: 2004-11-01
• 期刊: FREE RADICAL BIOLOGY AND MEDICINE
• 影响因子: 7.4
• 作者: Okai, Y;Sato, EF;Inoue, M
• 通讯作者: Inoue, M