Analysis and Development of Anti-inflammatory peptide derived from Annexin 1

膜联蛋白1是膜联蛋白超家族中的一员，它以钙依赖的方式与膜磷脂结合。这些蛋白质的N-末端结构域具有独特的性质，在不同的细胞中展示其特定的功能。我们以前报道过，膜联蛋白1在中性粒细胞中含量特别丰富，占胞浆蛋白的3%。由于在炎症组织中发现的大部分膜联蛋白1与中性粒细胞有关，该蛋白可能在炎症过程的调节中发挥重要作用。为了分析膜联蛋白1的生理功能，我们建立了人膜联蛋白1转基因小鼠。转基因小鼠的身体状况正常。表型和繁殖。膜联蛋白1转基因小鼠的中性粒细胞产生的超氧阴离子略有减少。血液中的中性粒细胞数量也有所下降。膜联蛋白1转基因小鼠显著阻断脂多糖(LPS)刺激的中性粒细胞迁移。此外，我们还发现，在膜联蛋白1转基因小鼠中，酪蛋白诱导的中性粒细胞募集也受到抑制。在此基础上，我们将讨论膜联蛋白1在体内的生理作用。

Annexin 1 is a member of annexin superfamily proteins that bind to membrane phospholipids in a calcium-dependent manner. The N-terminal domains of these proteins have unique properties to exhibit their specific functions in various cells. We previously reported that annexin 1 is particularly abundant in neutrophils where it represents up to 3% of the cytosolic proteins. Because the majority of annexin 1 found in inflammatory tissues is associated with neutrophils, this protein might play important roles in the modulation of inflammatory processes. To analyze the physiological roles of annexin 1, we established human annexin1-transgenic mice. The transgenic mice had normal. phenotype and reproduction. Superoxide generation from neutrophils of annexin 1-transgenic mice slightly decreased. The population of neutrophils in blood also decreased. Annexin 1-transgenic mice dramatically blocks lipopolysaccharide (LPS)-stimulated neutrophil migration. Furthermore, we show that neutrophils recruitment induced by casein is suppressed also in annexin 1-transgenic mice. Based on such observations, physiological roles of annexin 1 in vivo will be discussed.