权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular pathological analysis of the ghrelin expression in H. Pylori associated diseases.

幽门螺杆菌相关疾病中生长素释放肽表达的分子病理学分析。

基本信息

批准号：
16590283
负责人：
SEKINE Ichiro
金额：
$ 2.24万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590283/
关键词：
Ghrelin Gastretomy H.Pylori Gastritis Appetite Growth hormon IGF-I BMI

项目摘要

There are contradictory reports on the relationship between Helicobacter pylori and circulating ghrelin. We sought to clarify the influence of H.pylori infection on gastric and plasma ghrelin dynamics in humans. METHODS : Using endoscopic biopsies from the corpus of 56 H.pylori-infected patients and 25 uninfected subjects, ghrelin mRNA expression levels and gastric ghrelin peptide contents were measured by real-time polymerase chain reaction and radioimmunoassay, respectively. We also measured plasma ghrelin concentrations and analyzed the numbers of ghrelin immunoreactive cells in the fundic gland area. Fifty-one patients with H. pylori infection were treated with a 7-day triple therapy consisting of lansoprazole, clarithromycin, and amoxicillin. RESULTS : The gastric ghrelin mRNA expression level of H.pylori-positive patients (1.64 +/- 1.27 in arbitrary units) was significantly lower than in H.pylori-negative subjects (4.87 +/- 4.1, p<0.0001). A similar trend was noted for ghrelin pe … More ptide contents (31.2 +/- 27.5 vs 81.2 +/- 64.1 ng/mg protein, respectively, p < 0.0001). There was no significant difference in the number of ghrelin immunoreactive cells/mm^2 in terms of H. plyori status. Plasma ghrelin concentrations in H.pylori-infected patients (144.6 +/- 7.8.8 fmol/ml) were significantly lower than in uninfected subjects (196.1 +/- 97.2, p < 0.05) and increased following cure of the infection. Plasma ghrelin levels correlated positively with the expression levels of ghrelin mRNA (r=0.583, p<0.0001) and peptide products (r=0.574, p<0.0001). There was a significant stepwise decrease in gastric ghrelin mRNA expression (p<0.05), peptide contents (p<0.01) and density of ghrelin immunoreactive cells (p<0.05) with progression of histological severity of glandular atrophy in the corpus. The histological severity of chronic inflammation also negatively influenced the ghrelin mRNA expression (p<0.001) and peptide production (p<0.005). CONCLUSIONS : H.pylori infection has a negative impact on gastric and plasma ghrelin dynamics. Chronic inflammatory and atrophic changes associated with the infection may affect gastric ghrelin biosynthesis and contribute to the low circulating levels.The patient with total gastrectomy or severe atrophic gastritis shows body weight reduction, anorexia, osteomalasia, etc. The ghrelin supply treatment might be beneficial effect for above symptoms as a kind of somatopause Less

关于幽门螺杆菌和循环胃饥饿素之间的关系有相互矛盾的报道。我们试图阐明幽门螺杆菌感染对人类胃和血浆ghrelin动力学的影响。方法：采用实时荧光定量聚合酶链反应和放射免疫法分别检测了56例幽门螺杆菌感染患者和25例未感染者胃体组织中ghrelin mRNA表达水平和胃ghrelin肽含量。我们还测量了血浆ghrelin浓度，并分析了胃底腺区ghrelin免疫反应细胞的数量。51例H.幽门螺杆菌感染的患者采用兰索拉唑、克拉霉素和阿莫西林三联疗法治疗7天。研究结果：幽门螺杆菌阳性患者的胃饥饿素mRNA表达水平（1.64 ± 1.27任意单位）显著低于幽门螺杆菌阴性受试者（4.87 ± 4.1，p<0.0001）。生长激素释放肽也有类似的趋势 ...更多信息 ptide含量（分别为31.2 +/-27.5vs 81. 2 +/-64.1ng/mg蛋白，p < 0.0001）。根据H，每平方毫米的ghrelin免疫反应细胞数没有显著差异。无菌状态。幽门螺杆菌感染患者的血浆ghrelin浓度（144.6 +/- 7.8.8 fmol/ml）显著低于未感染受试者（196.1 +/- 97.2，p < 0.05），并且在感染治愈后升高。血浆ghrelin水平与ghrelin mRNA（r=0.583，p<0.0001）和肽产物（r=0.574，p<0.0001）的表达水平呈正相关。随着腺体萎缩程度的加重，胃Ghrelin mRNA表达（p<0.05）、肽含量（p<0.01）和Ghrelin免疫反应阳性细胞密度（p<0.05）呈明显的递减趋势。慢性炎症的组织学严重程度也对ghrelin mRNA表达（p<0.001）和肽产生（p<0.005）产生负面影响。结论：幽门螺杆菌感染对胃和血浆ghrelin动力学有负面影响。感染引起的慢性炎症和萎缩性改变可能影响胃Ghrelin的生物合成，导致循环水平降低。全胃切除术或严重萎缩性胃炎患者表现为体重减轻、厌食、骨质软化等。Ghrelin补充治疗可能对上述症状产生有益影响，作为一种躯体疾病。