权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Molecular Pathological Analysis of Digestive Cancers around Semipalatinsk Nuclear Testing Site

塞米巴拉金斯克核试验场周边消化道癌症的分子病理学分析

基本信息

批准号：
14406002
负责人：
SEKINE Ichiro
金额：
$ 4.29万
依托单位：
Nagasaki University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (B)
财政年份：
2002
资助国家：
日本
起止时间：
2002 至 2004
项目状态：
已结题

项目摘要

Since 1949,hundreds of nuclear tests had been conducted at Semipalatinsk Nuclear Testing Site(SNTS) in Kazakhstan, during the period of former USSR. Due to the secret system of former USSR, clarification of health effect in the population residing around SNTS is quite difficult. It is estimated that irradiated population around SNTS during the periods of nuclear testing has a certain risk of late effects of radiation exposure, especially malignancy. However it is quite difficult to clarify the real situation, due to the insufficient medical and epidemiological infrastructure in this area.On the other hand, molecular mechanism of radiation induced cancer has been extensively investigated in order to clarify the late effect of radiation in A-bomb survivors in Nagasaki and Hiroshima. It was reported that the incidence of stomach cancer was increased after 30 years of A-bomb. However, its mechanism has not been clarified. Based on these backgrounds, we promoted the molecular pathological joint research on the evaluation of stomach cancer around SNTS, in cooperation with the Department of Pathology of Semipalatinsk Medical Academy. Furthermore, we screened the expression of EBV-encoded small RNA-1(EBER-1) by in situ hybridization, in order to evaluate the incidence of virus-induced stomach cancer in this area, and showed that the positive EBER-1 was observed in 10.8% of Russian and 8.9% of Asian population. These results suggest that the incidence of stomach cancer caused by EBV infection is not a significant confounding variable for the evaluation of radiation induced cancer, since the ratio of EVB infection of this cohort is not significantly different between Western countries and Japan. In future, we plan to develop the molecular pathological studies targeted p53 and p21, in addition to the introduction of new technologies, such as Comparative Genomic Hybridization(CGH).

自1949年以来，在前苏联时期，在哈萨克斯坦塞米巴拉金斯克核试验场进行了数百次核试验。由于前苏联的秘密制度，对SNTS周围人口的健康影响的澄清是相当困难的。据估计，核试验期间SNTS周围的受辐射人群具有一定的辐射暴露后期效应风险，特别是恶性肿瘤。然而，由于该地区的医疗和流行病基础设施不足，很难澄清实际情况。另一方面，为了阐明长崎和广岛原子弹爆炸幸存者的后期效应，人们对辐射致癌的分子机制进行了广泛的研究。据报道，原子弹爆炸30年后胃癌的发病率增加了。然而，其机制尚未明确。在此背景下，我们与塞米巴拉金斯克医学院病理科合作，开展了围绕SNTS评价胃癌的分子病理学联合研究。此外，我们通过原位杂交筛选ebv编码的小RNA-1（EBER-1）的表达，以评估该地区病毒诱导胃癌的发病率，结果显示，EBER-1阳性在10.8%的俄罗斯人群和8.9%的亚洲人群中观察到。这些结果提示，EBV感染引起的胃癌发病率并不是评估辐射诱发癌的重要混杂变量，因为该队列中EVB感染的比例在西方国家和日本之间没有显著差异。未来，我们计划在引入比较基因组杂交（CGH）等新技术的基础上，进一步开展针对p53和p21的分子病理学研究。