Roles of BCR signaling mediated by PLC-γ2 on memory response

PLC-γ2介导的BCR信号对记忆反应的作用

• 批准号: 16590414
• 负责人: HIKIDA Masaki
• 金额: $ 2.3万
• 依托单位: RIKEN
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590414/
• 关键词: PLC-γ2; IgG1; memory response; class switch; antigen receptor; signal transduction; signaling; conditional knock-out; IgGl; BCRシグナル; Cre recombinase; B細胞; リンパ球分化

We have crossed the PLC-γ2 conditional knock-out mice with IgG1-Cre expressing mice In order to clarify the roles of PLC-γ2 on memory response. In the B cells from IgG1-Cre mice, only the cells those express γ1 germline transcript express Cre recombinase. For this reason, B cells differentiate normally to mature stage and only those which responded to antigen and switched to IgG1+ express cre recombinase to abolish PLC-γ2 expression. This will make it possible to analyze the role of PLC-γ2 in the IgG1 memory response in comparison with response of other IgG class.We first examined whether PLC-γ2 gene is efficiently deleted in IgG1 switched cells in vitro or not, and found that it is the case in the mice we have established. Another finding is that IgG1 titer in those mice is markedly decreased in these mice. Together with these results, it is strongly suggested that PLC-γ2 is playing a pivotal role in memory responses.

我们已经用表达小鼠的IgG1-cre跨越了PLC-γ2条件敲除小鼠，以阐明PLC-γ2在记忆反应中的作用。在来自IgG1-CRE小鼠的B细胞中，只有那些表达γ1种系的细胞表达CRE重组酶。因此，B细胞正常区分为成熟阶段，并且只有对抗原反应并切换到IgG1+ Express CRE重组酶的那些阶段，以废除PLC-γ2的表达。与其他IgG类的反应相比，这将使PLC-γ2在IgG1记忆响应中的作用成为可能。我们首先检查了PLC-γ2基因在体外是否有效地在IgG1转换细胞中有效地删除，并发现在我们已经建立的小鼠中是这种情况。另一个发现是这些小鼠中这些小鼠的IgG1滴度明显减少。与这些结果一起，强烈建议PLC-γ2在记忆响应中起关键作用。

项目成果

Regulation of phospholipase C-γ2 networks in B lymphocytes

• DOI: 10.1016/s0065-2776(05)88003-4
• 发表时间: 2005-01-01
• 期刊: ADVANCES IN IMMUNOLOGY, VOL 88
• 作者: Hikida, M;Kurosaki, T
• 通讯作者: Kurosaki, T