Proteome analysis of heterogeneity of intrahepatic biliary epithelial cells

值得注意的是，微阵列技术几乎达到了一个顶峰。建立进一步分析和管理来自微阵列技术的大量数据是目前最优先考虑的问题。胆管细胞的异质功能调节着胆道上皮的分泌、增殖和凋亡的病理生理活动。微阵列分析显示了两种小鼠胆管细胞的不同表达谱，分别称为小和大。两种胆管细胞系的特征，部分根据基因本体分类，表明了每种细胞系的特定生理作用。也就是说，大胆管细胞的特点是“运输”和“免疫/炎症反应”。与大胆管细胞相比，小胆管细胞具有有限的生理功能能力和特定分子（如Eph受体）的增殖/迁移潜力，与间充质细胞相当。组学研究将有助于了解胆管细胞的异质性。在目前的研究中，我们通过1)建立手术获得的肝组织分离技术，2)通过转染SV40大T抗原建立新的培养方法，3)使用egfp转基因小鼠建立干细胞移植模型，为胆管细胞蛋白质组学研究进行了基础条件研究。利用这些新方法，可以进一步评估胆管细胞的特异性表达谱。

It is remarkable that microarray technologies have nearly reached a pinnacle. Establishment of further analysis and management of enormous data derived from microarray technology is currently the highest priority. The heterogeneous functions of cholangiocytes regulate the pathophysiology of the biliary epithelium regarding secretory, proliferative and apoptotic activities. Distinct expression profiles of two murine cholangiocytes lines, termed small and large were revealed by microarray analysis. The features of the two cholangiocyte cell lines, categorized partly according to gene ontology, indicate the specific physiological role of each cell lines. Namely large cholangiocytes are characterized as "transport" and "immune/inflammatory responses". In contrast to large, small cholangiocytes are associated with properties of limited physiological functional ability and proliferating/migrating potential with specific molecules like Eph receptors, comparable to mesenchymal cells. 'Omic study will be of great help to understanding the heterogeneiety of cholangiocytes. In the current study, we have performed the basic conditioning study for proteomic study of cholangiocyte by 1)establishing isolation technique from surgically obtained liver tissue, 2)developing novel culture methods by transfection of SV40 large T antigen, 3)developing stem cell transplantation model using EGFP-transgenic mice. With these novel methods, further evaluation of cholangiocytes specific expression profile could be determined.