Air-liquid interface microenvironment and its effects on growth and differentiation of mucosal epithelial cells of alimentary tract : its possible roles as a luminal factor

气液界面微环境及其对消化道粘膜上皮细胞生长和分化的影响：其作为管腔因子的可能作用

• 批准号: 16590604
• 负责人: TODA Shuji
• 金额: $ 1.47万
• 依托单位: Saga University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590604/
• 关键词: Air-liquid interface; Luminal factor; mucosal epithelium of alimentary tracts; Growth; Differentiation; Apoptosis; Culture; Survival; アポトーシ

Air-liquid interface (ALI) is a microenvironment for mucosal surface of alimentary tracts. We have shown that ALI microenvironment promotes the survival, growth and differentiation of gastric mucosal epithelium. In this study, we propose that ALI is a luminal factor of gastric mucosal epithelium. Our present project has demonstrated that ALI promoted the survival, proliferation and differentiation of mucosal epithelium of small and large intestines, while ALI did not affect the apoptosis. The ALI-promoted growth was intimately associated with MAP kinase cascade activation. The ALI-induced functional differentiation was closely related to the increased expression of aquaporin and pendrin molecules. These results suggest that ALI is an important luminal factor for mucosal epithelial cell types of alimentary tracts.

气液界面(ALI)是消化道粘膜表面的微环境。我们发现ALI微环境促进了胃粘膜上皮细胞的存活、生长和分化。在本研究中，我们认为ALI是胃粘膜上皮细胞的腔因子。我们目前的研究表明，ALI促进了小肠和大肠粘膜上皮细胞的存活、增殖和分化，而ALI不影响细胞的凋亡。ALI促进的生长与MAPK级联激活密切相关。ALI诱导的功能分化与水通道蛋白和侧膜蛋白分子表达增加密切相关。这些结果表明，ALI是影响消化道粘膜上皮细胞类型的重要因素。