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Arole of cell membrane microdomains in air-liquid interface-induced invasive growth of laryngeal carcinoma cells

细胞膜微区在气液界面诱导喉癌细胞侵袭性生长中的作用

基本信息

批准号：
18591871
负责人：
TODA Shuji
金额：
$ 2.13万
依托单位：
Saga University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2006
资助国家：
日本
起止时间：
2006 至 2007
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591871/
关键词：
Air-liquid interface Laryngeal cancer Sqaumous cell carcinoma Cell membrane microdomains Caveolin Growth Invasion Cholesterol inhibitor 咽頭癌

项目摘要

Air-liquid interface (ALI) promotes the invasive growth of squamous cell carcinoma (SCC) cells of the larynx in vitro. ALI-induced invasive growth of SCC cells is related to the following events. 1. The cell membrane microdomain caveolin is considered to be activated in SCC cells by ALI stimulation. 2. In ALI-induced invasive growth of SCC cells, caveolin, Ras/MAPK pathway molecules, MMP and filamin Aare enhanced, but it is unclear whether caveolin activation is associated with the enhancement of Ras/MAPK pathway molecules, MMP and filamin A 3. Cholesterol inhibitor is considered to prohibit ALI-induced invasive growth of SCC cells through caveolin inactivation that may induce the downregulation of Ras/MAPK pathway molecules, MMP and filamin A 4. ALI promotes the invasive growth of SCC cells in their planting nude mice, but ALI does not affect the metastasis of SCC cells. SCC cells in vivo express the cell membrane microdomain caveolin, but the inhibition of caveolin expression does not affect the invasive growth of SCC cells, suggesting that caveolin may be a responsible factor for the invasive growth of SCC cells in vivo. 5. Cholesterol inhibitor does not affect the growth, invasion and metastasis of SCC cells in their planting nude mice.

气液界面(ALI)促进喉鳞状细胞癌(SCC)细胞体外侵袭性生长。ALI诱导的SCC细胞侵袭性生长与以下事件有关。1.细胞膜微区小窝蛋白被认为在ALI刺激下在SCC细胞被激活。2.在ALI诱导的SCC细胞侵袭性生长过程中，小窝蛋白、RAS/MAPK途径分子、基质金属蛋白酶和细丝蛋白A3的表达增强，但目前尚不清楚小窝蛋白激活是否与RAS/MAPK途径分子、基质金属蛋白酶和细丝蛋白A3的增加有关。胆固醇抑制剂通过抑制小窝蛋白失活来抑制ALI诱导的SCC细胞侵袭生长，这种失活可能导致RAS/MAPK途径分子、基质金属蛋白酶和细丝蛋白A4的下调。ALI促进裸鼠种植的SCC细胞的侵袭生长，而ALI不影响SCC细胞的转移。体内SCC细胞表达细胞膜微域小窝蛋白，但抑制小窝蛋白的表达并不影响SCC细胞的侵袭性生长，提示小窝蛋白可能是体内SCC细胞侵袭性生长的负责因素。5.胆固醇抑制剂不影响人鳞状细胞癌裸鼠移植瘤的生长、侵袭和转移。