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CYR61 Expression and Function in rapidly progressive glomerulonephritis

CYR61 在急进性肾小球肾炎中的表达和功能

基本信息

批准号：
16590792
负责人：
KOHDA Yukimasa
金额：
$ 2.11万
依托单位：
Kumamoto University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590792/
关键词：
RPGN CYR61 glomerulonephritis 急速進行性糸球体腎炎慢性糸球体腎炎

项目摘要

To certify the expression of CYR61 protein, 174 human renal biopsy samples were immuno-stained with anti-CYR61 antibody. 19 of 174 cases were rapidly progressive glomerulonephritis. 6 (30%) of 19 rapidly progressive glomerulonephritis showed positive reaction with anti-CYR61 Antibody. 4 cases showed positive reaction in cellular Crescents. 2 cases showed positive reaction in tubles. 25 (16.1%) of 155 chronic glomerulonephritis samples showed positive reaction with anti-CYR61 antibody. No reaction was seen in glomeruli of chronic glomerulonephritis samples. 25 positive samples showed the reaction in tubles. Serum creatinine was 7.2mg/dl in the patients of "crescent positive stained RPGN", and 4.3mg/dl in "crescent negative stained RPGN". There were no differences between both groups. In chronic Glomerular samples, CYR61 positive group had more severe interstitial damages compare to CYR61 negative group. But there were any clinical differences in both groups.Because of cross reactivity o … More f antibodies, CYR61 expression was checked by another method, RT-PCR. mRNA was extracted from frozen renal biopsy samples. Small amount of renal biopsy samples were quickly frozen at the time of renal biopsy for immuno-fluorescent staining. Several frozen tissue were cut in 5μm thickness and quickly fixed with 70% ethanol to remove OCT compound. After 5 minutes, tissue sections were quickly air dried and RNA was extracted by 4M GTC solution. RT reaction was performed and PCR was performed with this cDNA. By regular 35cycle PCR, CYR61 mRNA products were seen. By real time PCR, there was no relation between the clinical future and CYR expression. Several reason of this negative result were the quality of mRNA, number of samples, especially less number of rapidly progressive glomerulonephritis.To determine the urinaly expression of CYR61, sensitivity of Western Blot was not enough to see CYR61. Sample collection and concentration method should be changed.The Expression of CYR61 was seen in severe glomerulonephritis, rapidly progressive glomerulonephritis. Especially the reaction was seen in crescents in rapidly progressive glomerulonephritis samples. So CYR61 will perform some reaction in human rapidly progressive glomerulonephritis. Less

对174例人肾活检标本进行了抗CYR61抗体免疫组织化学染色，以证实CyR61蛋白表达。174例中19例为急进性肾小球肾炎。19例急进性肾小球肾炎中有6例(30%)抗-CYR61抗体阳性。4例细胞新月体呈阳性反应。2例输卵管呈阳性反应。155例慢性肾小球肾炎标本中有25例(16.1%)抗CyR61抗体呈阳性反应。慢性肾小球肾炎标本肾小球内未见反应。25份阳性标本在试管中呈阳性反应。“新月型阳性染色RPGN”患者血清肌酐为7.2 mg/dl，“新月型阴性染色RPGN”患者为4.3 mg/dl。两组间无差异。在慢性肾小球标本中，CYR61阳性组的间质损害较阴性组严重。但两组在临床上无差异。由于…的交叉反应用另一种方法--RT-PCR检测更多的抗体。从冰冻的肾活检标本中提取mRNA。取少量肾活检标本，在肾活检时快速冷冻，进行免疫荧光染色。冰冻组织切成5μm厚，用70%乙醇快速固定，去除OCT化合物。5min后，快速风干组织切片，用4M GTC溶液提取RNA。用该基因进行RT反应和聚合酶链式反应。常规35周期聚合酶链式反应可检测到CYR61mRNA的表达。实时荧光定量聚合酶链式反应显示临床预后与Cyr表达无关。造成这一阴性结果的几个原因是mRNA的质量、样本数量，特别是快速进行性肾炎的数量较少。要确定CYR61的尿液表达，Western Blot的灵敏度不足以看到CyR61。应改变标本采集和浓缩方法，在重度肾小球肾炎、急进性肾小球肾炎中可见CyR61的表达。尤其是在急进性肾小球肾炎的新月体中可见这种反应。因此，在人类急进性肾小球肾炎中，CYR61会产生一定的反应。较少

项目成果

期刊论文数量（19）

专著数量（0）

科研奖励数量（0）

会议论文数量（0）

专利数量（0）

Differential Effects of Hyperosmolality on Na-K-ATPase and Vasopressin-Dependent cAMP Genaration in the Medullary Theick Ascending Limb and Outer Medullary Collectiong Duct.

高渗透压对髓质升肢和外髓集合管中 Na-K-ATP 酶和加压素依赖性 cAMP 生成的不同影响。

DOI：
发表时间：
2005
期刊：
Hypertens Res 8(8)
影响因子：
0
作者：
Sakuma Y;Nonoguchi H;Takayama M;Yang T;Terada Y;Inoue T;Nakayama Y;Kohda Y;Sakaki S;Tomita K.
通讯作者：
Tomita K.

Regulation of aquaporin-2 by metabolic acidosis

代谢性酸中毒对 aquaporin-2 的调节