Novel Technologies for the Purification of Recombinant Proteins

大规模纯化重组蛋白的技术是生物技术产业的基本要求。本项目旨在整合分子生物识别中的两个概念。固定化金属离子亲和层析的新模式将与新型肽亲和柄的选择联系在一起，这些肽亲和柄可以通过基因工程方法插入到由生物技术程序产生的蛋白质中。接下来的方法允许在分离目标蛋白后去除这些手柄。因此，这个项目可能会导致用于人类治疗的重组蛋白的制造取得相当大的进步。

Availability of enabling technologies for the large-scale purification of recombinant proteins is an essential requirement of the biotechnology industry. This project aims to integrate two concepts in molecular biorecognition. New modalities of immobilised metal ion affinity chromatography will be linked with the selection of new types of peptide affinity handles that can be inserted by genetic engineering methods into proteins produced by biotechnological procedures. The approach followed then allows these handles to be removed following isolation of the target protein. This project could thus lead to a considerable advance in the manufacture of recombinant proteins intended for human therapy.