Novel Technologies for the Purification of Recombinant Proteins

重组蛋白纯化新技术

• 批准号: LP0561064
• 负责人: Em/Prof Milton Hearn AM FTSE
• 金额: $ 81.28万
• 依托单位: Monash University
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2005
• 资助国家: 澳大利亚
• 起止时间: 2005-03-30 至 2008-12-31
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0561064
• 关键词: Novel; Technologies; Purification; Recombinant; Proteins

Availability of enabling technologies for the large-scale purification of recombinant proteins is an essential requirement of the biotechnology industry. This project aims to integrate two concepts in molecular biorecognition. New modalities of immobilised metal ion affinity chromatography will be linked with the selection of new types of peptide affinity handles that can be inserted by genetic engineering methods into proteins produced by biotechnological procedures. The approach followed then allows these handles to be removed following isolation of the target protein. This project could thus lead to a considerable advance in the manufacture of recombinant proteins intended for human therapy.

获得大规模纯化重组蛋白的使能技术是生物技术工业的基本要求。该项目旨在整合分子生物识别中的两个概念。固定化金属离子亲和层析的新模式将与选择新型多肽亲和手柄相联系，这些手柄可以通过基因工程方法插入到通过生物技术程序产生的蛋白质中。随后的方法允许在分离目标蛋白后移除这些手柄。因此，该项目可能导致用于人类治疗的重组蛋白制造方面的相当大的进步。