Development of highly sensitive assay for TSH receptor antibody (TRAb) ; simultaneous measurements of TSAb, TSBAb, and TBII

开发高灵敏度 TSH 受体抗体 (TRAb) 检测方法；

• 批准号: 16590911
• 负责人: TAKASU Nobuyuki
• 金额: $ 2.3万
• 依托单位: University of the Ryukyus
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16590911/
• 关键词: TSAb; TSBAb; TBII; TRAb; Graves' disease; Hashimoto's thyroiditis; hyperthyroidism; hypothyroidism; TSH受容体; TSH受容体抗体; 超高感度TSAb

TSH receptor antibody (TRAb) causes Graves' disease. TSH-binding inhibitor immunoglobulin (TBII) and thyroid stimulating antibody (TSAb) have been measured as TSH receptor antibody (TRAb) to diagnose Graves' disease. TBII is measured as a receptor assay and TSAb as a stimulator assay. TSAb stimulates the thyroid gland and causes Graves' hyperthyroidism. TSAb reflects thyroid stimulating antibody (TSAb)-activity, but TBII does not necessarily do so. TBII may reflect the activity of TSH-stimulation blocking antibody (TSBAb). TSBAb causes hypothyroidism. TBII has been widely used to diagnose Graves' disease.The aim of this study is to develop highly sTSAb (sensitive thyroid-stimulating antibody)-assay. We also intended to develop highly sensitive assay for TSH receptor antibody (TRAb), which would measure TSAb, TSBAb, and TBII, simultaneously.We developed a highly sensitive TSAb (sTSAb) assay. We are now developing new assay to measure TSAb, TSBAb, and TBII, simultaneously.

TSH受体抗体（TRAb）导致Graves病。TSH结合抑制剂免疫球蛋白（TBII）和甲状腺刺激抗体（TSAb）已被测量作为TSH受体抗体（TRAb）诊断Graves病。TBII作为受体测定法测量，TSAb作为刺激物测定法测量。TSAb刺激甲状腺并导致Graves'甲状腺功能亢进症。TSAb反映甲状腺刺激抗体（TSAb）活性，但TBII不一定如此。TBII可反映TSH刺激阻断抗体（TSBAb）的活性。TSBAb会导致甲状腺功能减退。TBII已被广泛应用于Graves病的诊断，本研究旨在建立一种高灵敏度的sTSAb检测方法。本研究还建立了一种高灵敏度的TSH受体抗体（TRAb）检测方法，可同时检测TSAb、TSBAb和TBII。我们现在正在开发新的检测方法，以同时测量TSAb，TSBAb和TBII。

项目成果

Genome-wide linkage analysis of type 2 diabetes mellitus reconfirms the susceptibility locus on 11p13-p12 in Japanese

• DOI: 10.1007/s10038-004-0199-3
• 发表时间: 2004-11-01
• 期刊: JOURNAL OF HUMAN GENETICS
• 影响因子: 3.5
• 作者: Nawata, H;Shirasawa, S;Sasazuki, T
• 通讯作者: Sasazuki, T

Acute brain injury in hypoglycaemia-induced hemiplegia

• DOI: 10.1111/j.1464-5491.2004.01185.x
• 发表时间: 2004-06-01
• 期刊: DIABETIC MEDICINE
• 影响因子: 3.5
• 作者: Shirayama, H;Ohshiro, Y;Takasu, N
• 通讯作者: Takasu, N

Acute brain injury in hypglycaemia-induced hemiplegia.

低血糖引起的偏瘫中的急性脑损伤。

• 发表时间: 2004
• 期刊: Diabet Med 21 (6)
• 作者: Shirayama H;Takasu N
• 通讯作者: Takasu N

Sensitive thyroid-stimulating antibody assay with high concent-rations of polyethylene glycol for the diagnosis of Graves' disea

高浓度聚乙二醇敏感促甲状腺抗体检测诊断格雷夫斯病

• 发表时间: 2004
• 期刊: Clin Exp Pharmacol Physiol 31(5-6)
• 作者: Takasu N;Kamijo K;Sato Y;Yoshimura H;Nagata A;Ochi Y
• 通讯作者: Ochi Y

バセドウ病.In : わかりやすい免疫疾 (患宮坂信之編)

格雷夫斯病.In：易于理解的免疫疾病（宫坂伸之编辑）

• 发表时间: 2005
• 作者: Shibayama;H.;et al.;LaRue AC et al.;高須信行;高須信行;Kunishima S;Masuya M et al.;高須信行
• 通讯作者: 高須信行