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Signal transduction systems for thyroid cell proliferation and differentiation and their abnormalities in Graves' thyroid cells

甲状腺细胞增殖和分化的信号转导系统及其在格雷夫斯甲状腺细胞中的异常

基本信息

批准号：
62570509
负责人：
TAKASU Nobuyuki
金额：
$ 0.32万
依托单位：
Shinshu University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

We studied the signal transduction systems for thyroid cell proliferation and differentiation. We also studied the abnormalities in the signal transduction systems in the Graves' thyroid cells.1. Signal transduction systems for thyroid cell proliferation: PO(phosphatidyl inositol) turnover, IP3 production, cytoplasmic free calcium ([Ca^<2+>]i), arachidonic acid release and PG (prostaglandins) production. EGF stiumulates porcine thyroid cell proliferation. However, the signals for the thyroid cell proliferation were not known. We found that EGF induces PI turnover, produces IP3, and increases [Ca^<2+>]i, EGF also releases arachidonic acid from phosphatidyl inositol and stimulates PG production. The results indicate that PI turnover, IP3 production, [Ca^<2+>]i increase, arachidonic acid release and PG production play important roles for thyroid cell proliferation.2. Signal transduction systems for thyroid cell differentiation: cAMP mediated processes. TSH differentiates porcine thyroid cells. TSH stimulate cAMP production, follicle formation and thyroid hormone synthesis. Cyclic AMP plays important roles for these processes. TSH stimulates thyroid cell differentiation through cAMP-mediated processes.3. Abnormalities in the Graves' thyroid cells. There are some abnormalities in the cAMP and PG production systems in the Graves thyroid cells. These abnormalities may play some role in the pathogenesis of Graves' disease.Using cultured thyroid cells, we revealed the signal transduction systems for thyroid cell proliferation and differentiation. We also revealed the abnormalities in the signal transduction systems in the Graves' thyroid cells.

我们研究了甲状腺细胞增殖和分化的信号转导系统。我们还研究了Graves‘s甲状腺细胞信号转导系统的异常。甲状腺细胞增殖的信号转导系统：PO(磷脂酰肌醇)代谢、IP3产生、胞浆游离钙([Ca^&lt；2+&gt；]i)、花生四烯酸释放和前列腺素(PG)产生。EGF抑制猪甲状腺细胞增殖。然而，甲状腺细胞增殖的信号尚不清楚。我们发现，EGF诱导PI代谢，产生IP3，增加[Ca^&lt；2+&gt；]i，EGF还能从磷脂酰肌醇中释放花生四烯酸，刺激PG的产生。结果表明，PI周转、IP3生成、[Ca~(2+)]i升高、花生四烯酸释放和PG生成在甲状腺细胞增殖中起重要作用。甲状腺细胞分化的信号转导系统：cAMP介导的过程。TSH可分化猪甲状腺细胞。TSH刺激cAMP生成、卵泡形成和甲状腺激素合成。环AMP在这些过程中起着重要的作用。TSH通过cAMP介导的过程促进甲状腺细胞分化。格雷夫斯家的甲状腺细胞异常。Graves甲状腺细胞内cAMP和PG产生系统存在异常。这些异常可能在Graves病的发病机制中起一定作用。利用培养的甲状腺细胞，我们揭示了甲状腺细胞增殖和分化的信号转导系统。我们还发现了Graves‘s甲状腺细胞信号转导系统的异常。