Rapid genetic diagnosis with the TRC (transcription-reverse transcription concerted reaction) system for cancer micrometastasis^1

利用TRC（转录-逆转录协同反应）系统对癌症微转移进行快速基因诊断^1

• 批准号: 16591310
• 负责人: FUJIWARA Yoshiyuki
• 金额: $ 2.3万
• 依托单位: Osaka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591310/
• 关键词: genetic diagnosis; micrometastasis; TRC; RNA amplification; gastric cancer; peritoneal lavage; TRC診断; 微小転移診断; CEA; AFP; SCC; リンパ節転移; TRC; PCR; 迅速転移診断

Detection of cancer micrometastases that are missed by conventional cytological and histopathological examination is required for improvement of cancer therapy. The aim of this study was to establish a rapid and practical genetic assay to detect micrometastasis in gastric cancer and to assess its clinical significance with respect to prognosis.A novel RNA amplification system with TRC reaction (transcription-reverse transcription concerted reaction) was introduced for quantitative detection of CEA (carcinoembryonic antigen) mRNA, which is widely used as a molecular marker for cancer micrometastasis. Peritoneal lavage fluid specimens and lymph nodes collected from cancer surgery were subjected to the assay, and the clinical significance of the results in prediction of recurrence and survival was examined.The quantification, sensitivity, and reproducibility of the assay using the TRC reaction were equal to those of quantitative RT-PCR with LightCycler^<TM>. The most important advantage of the assay was its simplicity and rapidity. Molecular diagnosis of peritoneal lavage fluid by the TRC reaction significantly correlated with depth of invasion, peritoneal metastasis, clinical stage, overall survival and peritoneal recurrence-free survival.Molecular diagnosis of peritoneal lavage fluid with the TRC reaction could be a useful prognostic indicator for peritoneal recurrence and survival. Because the TRC reaction is more rapid and simple than RT-PCR as a format for detecting RNA sequences, it may enhance the genetic diagnosis of cancer micrometastasis and may contribute to cancer therapy.

传统的细胞学和组织病理学检查遗漏的癌症微转移的检测是改善癌症治疗所必需的。为建立一种快速、实用的胃癌微转移基因检测方法，并探讨其临床意义，本研究采用一种新的RNA扩增技术结合转录-逆转录协同反应（transcription-reverse transcriptionconcerted reaction，TRC），对胃癌微转移标志物癌胚抗原（carcinoembryonic antigen，CEA）mRNA进行定量检测。对肿瘤手术中收集的腹腔灌洗液和淋巴结标本进行检测，并对检测结果在预测复发和生存方面的临床意义进行了检验<TM>。该方法最重要的优点是简便、快速。TRC反应对腹腔灌洗液的分子诊断与浸润深度、腹膜转移、临床分期、总生存率和无腹膜复发生存率显著相关，TRC反应对腹腔灌洗液的分子诊断可能是预测腹膜复发和生存的一个有用指标。由于TRC反应作为检测RNA序列的形式比RT-PCR更快速和简单，因此它可以增强癌症微转移的基因诊断，并可能有助于癌症治疗。

项目成果

Rapid genetic diagnosis with the transcription-reverse transcription concerted reaction system for cancer micrometastasis

• DOI: 10.1245/aso.2004.12.043
• 发表时间: 2004-08-01
• 期刊: ANNALS OF SURGICAL ONCOLOGY
• 影响因子: 3.7
• 作者: Ishii, T;Fujiwara, Y;Monden, M
• 通讯作者: Monden, M

Clinical value of alterations in p73 gene, related to p53 at 1p36, in human hepatocellular carcinoma.

• DOI: 10.3892/ijo.24.2.441
• 发表时间: 2004-02
• 期刊: International journal of oncology
• 影响因子: 5.2
• 作者: T. Aoki;A. Miyamoto;S. Marubashi;H. Nagano;K. Umeshita;Y. Fujiwara;K. Dono;S. Nakamori;M. Sakon;M. Monden

微小転移診断の外科臨床応用

微转移诊断的外科临床应用

• 发表时间: 2004
• 期刊: 外科治療 91.1
• 作者: 藤原義之;他
• 通讯作者: 他

Expression level of valosin-containing protein (p97) is associated with prognosis of esophageal carcinoma

• DOI: 10.1158/1078-0432.ccr-0723-03
• 发表时间: 2004-08-15
• 期刊: CLINICAL CANCER RESEARCH
• 影响因子: 11.5
• 作者: Yamamoto, S;Tomita, Y;Aozasa, K
• 通讯作者: Aozasa, K

I. 診断 腹膜播種の腹腔鏡診断

一、诊断腹腔镜诊断腹膜播散

• 发表时间: 2004
• 期刊: 外科 66.8
• 作者: 藤原義之;他
• 通讯作者: 他