Identification of factors involving chemotherapy resistance in pancreatic cancer patients by Protein Chip system for developing prediction system of chemotherapy response.

通过蛋白质芯片系统识别胰腺癌患者化疗耐药相关因素，以开发化疗反应预测系统。

• 批准号: 16591296
• 负责人: AMBIRU Satoshi
• 金额: $ 1.79万
• 依托单位: Chiba University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591296/
• 关键词: pancreatic cancer; gemcitabine; protein chip; patient selection; Gemcitabine; 薬剤耐性因子; Annexin II; 化学療法

Measurement of serum Annexin II level using anti-Annexin II antibodyUsing 2-D gel electrophoresis, we compared the protein profiling expressed in pancreatic cancer cell line which is resistant against treatment with anti cancer drug, gemcitabine, established by treating cells with the drug for long time with wild type cell line. We identified Annexin II is highly expressed in cell line with chemo-resistance, but not in wild type cells. This indicated that Annexin II has a role in establishing chemo-resistance in cancer cells. This also suggested that measurement of serum Annexin II level in pancreatic cancer patients may indicate the prognosis of the patients with treatment of this medicine. We now established the system to measure the serum level of Annexin II using Protein Chip System (CYPHAGEN). This system resolve the peptide in the sample using laser spectrum and enable to measure the expression level in the sample. To obtain specifically the serum level of Annexin II, we modified … More the surface of the protein chip plate with anti-Annexin II antibody. Using this system, only Annexin II within the whole protein in the serum is captured with the antibody, and we are able to evaluate the concentration of this target protein with precise value even though the expression level is very low. We are now trying to measure Annexin II serum level of the pancreatic cancer patients and analyze whether this serum level is collate with patients prognosis after gemcitabine treatment. For this purpose, we already collected serum samples from patients before and after operation or chemotherapy.We also are trying to measure the urine levels of Annexin II using same system. Using these technique, we are now expecting that we can select the pancreatic cancer patients whose prognosis gemcitabine will improve and avoid the use of this medicine for patients who will not respond the therapy. To these patients, we will treat them with another medicine of other therapies, such as radiation. This system will provide the benefits for selecting the patients and will avoid the wasting precious time and cost of these patients. Less

用抗Annexin II抗体测定血清Annexin II水平我们用二维凝胶电泳比较了胰腺癌细胞株和野生型胰腺癌细胞株中Annexin II蛋白表达谱的差异。我们发现Annexin II在具有化学抗性的细胞系中高表达，而在野生型细胞中不表达。这表明膜联蛋白II在癌细胞中建立化学抗性中具有作用。提示胰腺癌患者血清Annexin II水平的测定可作为判断胰腺癌患者预后的指标。我们现在建立了使用蛋白质芯片系统（CYPHAGEN）测量膜联蛋白II血清水平的系统。该系统使用激光光谱解析样品中的肽，并能够测量样品中的表达水平。为了获得特异性的血清膜联蛋白II水平，我们修改了 ...更多信息 在蛋白质芯片板的表面涂上抗膜联蛋白II抗体。使用该系统，抗体仅捕获血清中整个蛋白质中的膜联蛋白II，即使表达水平非常低，我们也能够以精确值评估该靶蛋白的浓度。我们现在试图测量胰腺癌患者的Annexin II血清水平，并分析该血清水平是否与吉西他滨治疗后患者的预后相关联。为此，我们已经收集了手术或化疗前后患者的血清样本，并尝试使用相同的系统测量尿液中Annexin II的水平。使用这些技术，我们现在期望我们可以选择吉西他滨将改善预后的胰腺癌患者，并避免将这种药物用于治疗无效的患者。对于这些病人，我们会用另一种药物或其他疗法，例如放射治疗。该系统将为选择患者提供好处，并将避免浪费这些患者的宝贵时间和费用。少

项目成果

Identification of Apolipoporotein C-1 as a novel prognostic serum marker of pancreatic cancer by protein profiling and its role in cancer cell proliferation

通过蛋白质分析鉴定载脂蛋白 C-1 作为胰腺癌新型预后血清标志物及其在癌细胞增殖中的作用

• 期刊: Gastroenterology (in press)
• 作者: Togawa A;Takano S;Yoshitomi H;Sogawa K;Shida T;Kimura F;Shimizu H;Tomonaga T;Nomura F;Miyazaki M
• 通讯作者: Miyazaki M

Early biological and immune response to semi-identical liver or kidney allograft in miniature swine.

小型猪对半相同肝脏或肾脏同种异体移植物的早期生物和免疫反应。

• 发表时间: 2005
• 期刊: Transplant International 18
• 作者: Ohtsuka M;et al.
• 通讯作者: et al.

Identificatioll of Apolipoporotein C-1 as a novel prognostic serum marker of pancreatic cancerby protein profilingand its role in cancer cell proliferation

通过蛋白质分析鉴定载脂蛋白 C-1 作为胰腺癌新型预后血清标志物及其在癌细胞增殖中的作用

• 期刊: Gastroenterology in press
• 作者: Togawa A;et al.
• 通讯作者: et al.

Simple way to improve accuracy in diagnosis of quadrant inflammatory disease : How to a oid adverse laparotomy by using plain CT.

提高象限炎症性疾病诊断准确性的简单方法：如何使用平扫 CT 避免不良剖腹手术。

• 发表时间: 2005
• 期刊: Hepatogastroenterology 52
• 作者: Otsuka M;Ambiru S;Togawa A.
• 通讯作者: Togawa A.

Hyperbaric oxygen therapy for the treatment of postoperative paralytic ileus and adhesive intestinal obstruction associated with abdominal surgery : experience with 626 patients.

高压氧治疗用于治疗与腹部手术相关的术后麻痹性肠梗阻和粘连性肠梗阻：626 名患者的经验。

• 期刊: Hepato-Gastroenterology (in press)
• 作者: Ambiru S;Furuyama N;Aono M;Kimura F;Shimizu H;Yoshidome H;Miyazaki M;Shimada H;Ochiai T.
• 通讯作者: Ochiai T.