Significance of hRad9 expression in non-small cell lung cancer cells

非小细胞肺癌细胞中hRad9表达的意义

• 批准号: 16591395
• 负责人: MANIWA Yoshimasa
• 金额: $ 2.24万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591395/
• 关键词: DNA damage; lung cancer; hRad9; DNA複製; 細胞周期

hRad9 accumulation was observed in the nuclei of tumor cells in 33% of all nonsmall cell lung carcinoma (NSCLC) specimens examined, and elevated levels of phosphorylated hRad9 were found to be associated with the detection of phosphorylated Chk1. To the authors' knowledge, the current study was the first to describe the overexpression of hRad9 in surgically reseated tumor specimens and its phenotypic significance in tumor cells.Sequence analyses of the HRAD9 gene derived from 50 surgical specimens of lung adenocarcinoma were performed to investigate the status of this gene. Analysis of the coding regions indicated that there was no alteration in the HRAD9 sequence. Quantitative polymerase chain reaction of mRNA revealed that the gene was transcribed in all samples examined. However, these studies detected the presence of a nonsynonymous single nucleotide polymorphism (His239Arg) that occurred with a high frequency. This polymorphism appears to be associated with an increased risk of lung adenocarcinoma.

在所检查的所有非小细胞肺癌 (NSCLC) 样本中，33% 的肿瘤细胞核中观察到 hRad9 积累，并且发现磷酸化 hRad9 水平升高与磷酸化 Chk1 的检测相关。据作者所知，本研究首次描述了手术复位肿瘤标本中 hRad9 的过度表达及其在肿瘤细胞中的表型意义。对来自 50 个肺腺癌手术标本的 HRAD9 基因进行了序列分析，以调查该基因的状态。编码区分析表明HRAD9序列没有改变。 mRNA 的定量聚合酶链反应显示该基因在所有检查的样品中都被转录。然而，这些研究检测到存在高频率的非同义单核苷酸多态性（His239Arg）。这种多态性似乎与肺腺癌风险增加有关。

项目成果

Accumulation of hRad9 protein in the nuclei of non-small cell lung cancer (NSCLC) cells

hRad9 蛋白在非小细胞肺癌 (NSCLC) 细胞核中的积累

• 发表时间: 2005
• 期刊: Cancer 103・1
• 作者: Inoue;Y.;Yoshikawa R et al.;眞庭謙昌
• 通讯作者: 眞庭謙昌

Accumulation of hRad9 protein in the nuclei of non-small cell lung cancer (NSCLC) cells.

hRad9 蛋白在非小细胞肺癌 (NSCLC) 细胞核中的积累。

• 发表时间: 2005
• 期刊: Cancer 103-1
• 作者: Takamochi;K.;柳 秀憲 他;Yoshimasa Maniwa
• 通讯作者: Yoshimasa Maniwa

The His239Arg SNP of HRAD9 is associated with Lung Adenocarcinoma.

HRAD9 的 His239Arg SNP 与肺腺癌相关。

• 发表时间: 2006
• 期刊: Cancer 106
• 作者: Takata;M;Maniwa;Y*;Doi;T;Tanaka;Y;Okada;K;Nishio;W;Ohbayashi;C;Yoshimura;M;Hayashi;Y;Okita;Y;眞庭謙昌
• 通讯作者: 眞庭謙昌

Accumulation of hRad9 protein in the nuclei of nonsmall cell lung carcinoma cells

• DOI: 10.1002/cncr.20740
• 发表时间: 2005-01-01
• 期刊: CANCER
• 影响因子: 6.2
• 作者: Maniwa, Y;Yoshimura, M;Okita, Y
• 通讯作者: Okita, Y