Significance of hRad9 expression in non-small cell lung cancer cells

在33%的非小细胞肺癌（NSCLC）样本的肿瘤细胞核中观察到hRad9的积累，并且发现磷酸化hRad9水平的升高与磷酸化Chk1的检测相关。据作者所知，本研究首次描述了hRad9在手术复位肿瘤标本中的过表达及其在肿瘤细胞中的表型意义。本文对50例肺腺癌手术标本中的HRAD9基因进行了序列分析，以探讨该基因的状态。编码区分析表明，HRAD9序列未发生改变。mRNA的定量聚合酶链反应显示该基因在所有检测样本中均有转录。然而，这些研究发现了非同义单核苷酸多态性（His239Arg）的存在，这种多态性发生的频率很高。这种多态性似乎与肺腺癌的风险增加有关。

hRad9 accumulation was observed in the nuclei of tumor cells in 33% of all nonsmall cell lung carcinoma (NSCLC) specimens examined, and elevated levels of phosphorylated hRad9 were found to be associated with the detection of phosphorylated Chk1. To the authors' knowledge, the current study was the first to describe the overexpression of hRad9 in surgically reseated tumor specimens and its phenotypic significance in tumor cells.Sequence analyses of the HRAD9 gene derived from 50 surgical specimens of lung adenocarcinoma were performed to investigate the status of this gene. Analysis of the coding regions indicated that there was no alteration in the HRAD9 sequence. Quantitative polymerase chain reaction of mRNA revealed that the gene was transcribed in all samples examined. However, these studies detected the presence of a nonsynonymous single nucleotide polymorphism (His239Arg) that occurred with a high frequency. This polymorphism appears to be associated with an increased risk of lung adenocarcinoma.