Non-synonymous SNPs of DNA damage repair genes in lung cancer patients

肺癌患者DNA损伤修复基因的非同义SNP

• 批准号: 18591547
• 负责人: MANIWA Yoshimasa
• 金额: $ 2.6万
• 依托单位: Kobe University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2006
• 资助国家: 日本
• 起止时间: 2006 至 2007
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-18591547/
• 关键词: lung cancer; risk factor; DNA damage repair gene; single nucleotide polymorphism

In this study, we identified gene alterations in lung cancer patients, not from the perspective of acquired mutations caused by various external insults or damaging agents, but from the perspective of hereditary predisposition. We evaluated all the non-synonymous SNPs in the DNA damage repair genes that are present in the available databases, which should allow us to detect those that are closely related with the development of lung adenocarcinoma. Finally, we will organize the data statistically and systematically to facilitate the prediction of a patient's cancer risk. As a results, we confirmed that nonsynonymous SNPs (XRCCI: Arg194Trp, TDG: Gly199Ser, RAD9: His239Arg, POLδ1: Arg119His) were present significantly more frequently in lung cancer patients than in a control group. Alignment analysis revealed that all of the region that contained the SNPs conserved among vertebrates, and the allele frequency is less than 1 % in a normal control population. These findings suggest that this SNP may cause an alteration in the biological activity of the expressed protein. These SNPs may allow to predict the susceptibility of lung cancer in a healthy person.

在这项研究中，我们确定了肺癌患者的基因改变，不是从各种外部损伤或破坏剂引起的获得性突变的角度，而是从遗传易感性的角度。我们评估了现有数据库中存在的DNA损伤修复基因中的所有非同义SNPs，这将使我们能够检测出与肺腺癌发生密切相关的基因。最后，我们将统计和系统地组织数据，以便于预测患者的癌症风险。结果，我们证实了非同义SNP（XRCCI：Arg194Trp，TDG：Gly199Ser，RAD 9：His239Arg，POLδ1：Arg119His）在肺癌患者中比对照组显著更频繁地存在。比对分析表明，所有包含SNP的区域在脊椎动物中是保守的，并且在正常对照群体中等位基因频率小于1%。这些发现表明，该SNP可能导致表达蛋白的生物活性改变。这些SNPs可以预测健康人肺癌的易感性。

项目成果

DNA damage sensor protein hRad9, a novel molecular target for lung cancer treatment.

• DOI: 10.3892/or_00000108
• 发表时间: 2008
• 期刊: Oncology reports
• 影响因子: 4.2
• 作者: Takeshi Yuki;Y. Maniwa;T. Doi;K. Okada;W. Nishio;Y. Hayashi;Y. Okita

The His239Arg SNP of HRAD9 is associated with Lung Adenocarcinoma.

HRAD9 的 His239Arg SNP 与肺腺癌相关。

• 发表时间: 2006
• 期刊: Cancer 106
• 作者: Takata;M;Maniwa;Y*;Doi;T;Tanaka;Y;Okada;K;Nishio;W;Ohbayashi;C;Yoshimura;M;Hayashi;Y;Okita;Y;眞庭謙昌
• 通讯作者: 眞庭謙昌

Study of a relationship between DNA damage response proteins and lung cancer

DNA损伤反应蛋白与肺癌关系的研究

• 发表时间: 2006
• 作者: Maniwa;V
• 通讯作者: V

Non-synonymous. SNPs of DNA damage repair genes in lung cancer patients

非同义。

• 发表时间: 2006
• 作者: Maniwa;Y;et. al.
• 通讯作者: et. al.

Double-layered collagen gel hemisphere for cell invasion assay: Successful visualization and quantification of cell invasion activity

• DOI: 10.1080/15419060701557859
• 发表时间: 2007-01-01
• 期刊: CELL COMMUNICATION AND ADHESION
• 作者: Takata, Masahiko;Maniwa, Yoshimasa;Okita, Yutaka
• 通讯作者: Okita, Yutaka