Proteomics Analysis of Interactions Between Chaperonin 10 and Cell Surface Proteins

伴侣蛋白 10 与细胞表面蛋白相互作用的蛋白质组学分析

• 批准号: LP0561097
• 负责人: Prof Ross Smith
• 金额: $ 20.82万
• 依托单位: The University of Queensland
• 依托单位国家: 澳大利亚
• 项目类别: Linkage Projects
• 财政年份: 2005
• 资助国家: 澳大利亚
• 起止时间: 2005-02-14 至 2008-02-13
• 项目状态: 已结题
• 来源: https://dataportal.arc.gov.au/RGS/Web/Grants/LP0561097
• 关键词: Proteomics; Analysis; Interactions; Between; Chaperonin

Specific interactions at the cell surface may induce downstream biological responses. In the case of chaperonin 10, Cpn10, interaction at the cell surface results in immunomodulation of the inflammatory response. Cell surface proteins that interact with Cpn10 have not been identified. This project will use chemical crosslinking and proteomic techniques to identify cell surface proteins that interact with Cpn10 and structural features of Cpn10 involved in these interactions. These findings will providde leads for the development of immunomodulatory therapeutics based on Cpn10 interactions. Analytical technologies will be developed that are applicable to other interacting protein systems.

细胞表面的特异性相互作用可诱导下游生物反应。在伴侣蛋白10 Cpn10的情况下，在细胞表面的相互作用导致炎症反应的免疫调节。与Cpn10相互作用的细胞表面蛋白尚未鉴定。该项目将使用化学交联和蛋白质组学技术来鉴定与Cpn10相互作用的细胞表面蛋白以及参与这些相互作用的Cpn10的结构特征。这些发现将为基于Cpn10相互作用的免疫调节疗法的开发提供线索。将开发适用于其他相互作用蛋白质系统的分析技术。