Preparation of molecularly imprinted polymer sensor for blood level measurement of intravenous anesthetic.

用于静脉麻醉药血浓度测量的分子印迹聚合物传感器的制备

• 批准号: 16591549
• 负责人: IBUSUKI Shoichiro
• 金额: $ 2.18万
• 依托单位: University of Miyazaki
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591549/
• 关键词: molecularly imprinted polymer; propofol; quartz crystal microbalance; blood level; methacrylic acid; ethacrylic acid

The purpose of this research was preparation of a sensor for blood level measurement of intravenous anesthetic such as propofol. The molecularly imprinted polymers (MIPs) were employed as a propofol sensitive layer, and quartz crystal microbalances (QCM) were employed to determine the propofol concentration.In 2004, for practice of membrane preparation, the propofol selective MIPs were polymerized on the surface of slide glasses. Propofol (template), methacrylic acid (MAA, functional monomer), ethylene glyacol dimethacrylate (EGDMA, cross-linker) and 2,2'-Azobis(2.4-dimethyl valeronitrile) (initiator) were measured 1 : 6.5 : 8.5 : 0.4 (mol ratio, respectively) and dissolved in hexane. The mixture solution was painted thinly on the surface of slide glass and polymerized at 40℃ for 48 hours. It was very difficult to make the MIP into flat and thin layer. In 2005, we tried to improve the MIP layer preparation. The method of MIP polymerization was changed to precipitation polymerization to make MIP into microspheres. The MIP microspheres were binded onto slide glass or quartz crystal with PVC. However, it was still difficult to make MIP microspheres into flat/thin layer. Therefor, the property of MIP-QCM system could not be estimated. Additional improvement was needed

本研究旨在研制一种用于异丙酚等静脉麻醉药血药浓度测量的传感器。采用分子印迹聚合物（MIPs）作为异丙酚敏感层，石英晶体微天平（QCM）测定异丙酚浓度，并在2004年将MIPs聚合在载玻片表面，用于膜制备。将丙泊酚（模板）、甲基丙烯酸（MAA，功能单体）、乙二醇二甲基丙烯酸酯（EGDMA，交联剂）和2，2 '-偶氮双（2.4-二甲基戊腈）（引发剂）测量为1：6.5：8.5：0.4（摩尔比，分别）并溶解在己烷中。将该混合物溶液薄薄地涂在载玻片表面，并在40℃下聚合48小时。MIP很难制成平整的薄层。2005年，我们尝试改进MIP层制备。将MIP聚合方法改为沉淀聚合法，将MIP制成微球。用PVC将MIP微球固定在载玻片或石英晶体上。但MIP微球的制备仍存在一定的困难。因此，MIP-QCM系统的性质无法估计。需要进一步改进