Preparation of molecularly imprinted polymer sensor for blood level measurement of intravenous anesthetic.

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The purpose of this research was preparation of a sensor for blood level measurement of intravenous anesthetic such as propofol. The molecularly imprinted polymers (MIPs) were employed as a propofol sensitive layer, and quartz crystal microbalances (QCM) were employed to determine the propofol concentration.In 2004, for practice of membrane preparation, the propofol selective MIPs were polymerized on the surface of slide glasses. Propofol (template), methacrylic acid (MAA, functional monomer), ethylene glyacol dimethacrylate (EGDMA, cross-linker) and 2,2'-Azobis(2.4-dimethyl valeronitrile) (initiator) were measured 1 : 6.5 : 8.5 : 0.4 (mol ratio, respectively) and dissolved in hexane. The mixture solution was painted thinly on the surface of slide glass and polymerized at 40℃ for 48 hours. It was very difficult to make the MIP into flat and thin layer. In 2005, we tried to improve the MIP layer preparation. The method of MIP polymerization was changed to precipitation polymerization to make MIP into microspheres. The MIP microspheres were binded onto slide glass or quartz crystal with PVC. However, it was still difficult to make MIP microspheres into flat/thin layer. Therefor, the property of MIP-QCM system could not be estimated. Additional improvement was needed