Elucidation of receptors for salivary protein histatin in human gingival fibroblast

牙周病会导致与牙齿和口腔组织接触的口腔细菌。结果显示牙槽骨被破坏。这些是由炎症宿主细胞之间复杂的相互作用产生的。我们报道了来自牙周细菌的LPS或蛋白酶刺激人牙龈成纤维细胞（HGF）的组织破坏和炎症细胞因子的产生。组他汀类药物的功能是杀灭念珠菌活性和抑制龋齿细菌增殖。研究发现，组他汀类药物对艾滋病的表达减少。阐明了组他汀类药物对牙周细菌蛋白酶的失活作用和对HGF增殖的促进作用。当组抑素在口腔组织中的定位被调查时，我们可能会期望它们的功能。并研究了与组他汀类药物相互作用的分子的鉴定。用抗组蛋白抗体对口腔组织进行染色，发现组蛋白存在于腮腺、颌下腺、舌层颗粒和颊黏膜刺细胞层。组他汀类药物在腮腺和颌下腺中特异性表达。组他汀类药物可被转运到组织和细胞的细胞间隙或细胞内。组蛋白5是组蛋白家族的一员，与白色念珠菌的热休克蛋白（HSP） SSA 1/2p结合。结果显示抗菌作用。然后，我们研究了组蛋白是否与人类热休克蛋白相互作用。HSC70是HSPs的成员，与组蛋白相关，组蛋白结合到HSC70的肽结合结构域。组蛋白通过胞吞作用转运到细胞内，组蛋白/HSC70复合体转运到细胞核内。由于这在细胞热处理中是显著的，这表明组蛋白的易位依赖于HSC70。热休克蛋白的功能是蛋白质的结构形成，变性蛋白质的再折叠和防止凝析蛋白质。HSC70作为toll样受体的配体，与DNA复制和转录有关。提示HGF的增殖和存活与组蛋白有关。组抑素与细胞因子之间的相互作用尚未见报道。此外，组蛋白与热休克蛋白之间生理功能的详细研究似乎与组蛋白药物设计的发现有关。这可以应用于口腔疾病并应用于生殖领域。少

Periodontal diseases cause oral bacteria that contact with teeth and oral tissues. The results indicate the destruction of alveolar bones. These are generated by the complicated interaction between inflammatory host cells. We reported that the tissue destruction and production of inflammatory cytokines from human gingival fibroblast (HGF) were stimulated by LPS or protease derived from periodontal bacteria. Functions of histatins are a Candidacidal activity and a suppression of bacterial proliferation for caries. And it found the reduced expression of histatins for AIDS. We clarified about inactivation of proteases from periodontal bacteria and enhancement of proliferation in HGF by histatins. When the localization of histatins in a mouth organization was investigated, we may be expected the functions of those. And the identification of molecles that interact with histatins was investigated.When oral tissues were stained with anti-histatin antibody, histatins were existed in a parotid, … More a submandibular gland, tongue stratum granulosum and buccal mucosa prickle-cell layer. Histatins were specifically expressed in the parotid and the submandibular gland. And histatins may be transported into intercellular or intracellular space of tissues and cells.Histatin 5, a member of histatin family, bound to heat shock protein (HSP) SSA 1/2p from Candida albicans. The result showed antimicrobial action. Then, we investigated whether histatin was interacted with human HSPs. HSC70 that is a member of HSPs was associated with histatin, which bound to the peptide-binding domain of HSC70. Histatin was transported into the cells by the endocytosis and histatin/HSC70 complex was translocated into nuclei. Since this was remarkable at heat treatment of cells, it was suggested that the translocation of histatin is dependent on HSC70. The functions of HSPs are structural formation of proteins, refolding of denatured proteins and prevention of condensational proteins. And HSC70, in relation to a DNA replication and transcription, serves as a ligand of Toll-like receptor. These results suggested that the proliferation and survival of HGF was related with histatin. The interaction between histatins and cellular factors has not yet been reported. Moreover, it seems that detailed study for the physiological functions between histatin and HSP is connected with drug design discovery for histatin. And this could apply to the oral diseases and employ a field of reproduction. Less