Ets family expression in cultured cells from oral tissue

了解上皮细胞与成纤维细胞之间的相互作用及其在口腔组织中的信号转导特点，对于阐明口腔疾病的发病机制具有重要意义。Ets因子是一类重要的转录调节因子，在造血、血管生成、器官发生、肿瘤发生和神经元连接的特化中发挥关键作用。这些蛋白质主要在造血细胞中表达，然而，一些也在上皮细胞中表达。本研究探讨Ets基因家族、上皮特异性ets转录因子家族成员-1、-2和-3（ESE-1、-2和-3）在颊粘膜癌细胞系HO-1-N-1、上皮样细胞中的表达及调控。HO-1-N-1细胞本身在无刺激时也有ESE-1、ESE-2和ESE-3 mRNA的表达。IL-1α和IL-1β以剂量依赖性方式增加ESE-3 mRNA表达，但对ESE-1和ESE-2 mRNA表达无明显影响。佛波醇1/2-肉豆蔻酸酯13-乙酸酯（PMA）也以剂量依赖方式增加ESE-3 mRNA的表达，PMA促进的ESE-3 mRNA表达可被PKC抑制剂（双吲哚马来酰亚胺，BIS）和MEK 1/2抑制剂（U 0126）抑制。这些结果表明ESE-3可能是炎症过程中的重要决定因素。另外，HO-1-N-1细胞中PKC激活剂促进ESE-3 mRNA表达的作用是通过MEK 1/2途径实现的。

In order to clarify the mechanism of oral disease, it is quite important to know an interaction between epithelium and fibroblast and its characteristics on signal transduction in oral tissue. Ets factors constitute one important class of transcriptional regulators that play critical roles in hematopoiesis, angiogenesis, organogenesis, oncogenesis, and specification of neuronal connectivity. These proteins are expressed mainly in hematopoietic cells, however, some are also expressed in epithelial cells. The present study was investigated Ets gene family, Epithelium-specific ets transcription factor, family member-1,-2, and-3 (ESE-1,-2,and-3), expression and regulation in buccal mucosa carcinoma-derived cell line, HO-1-N-1,epithelial-like cell. HO-1-N-1 cell itself showed ESE-1,-2 and-3 mRNA expression in the absence of stimulant. IL-1α and IL-1β increased ESE-3 mRNA expression in a dose-dependent manner, however, they did not clearly alter ESE-1 and-2 mRNA expression. Phorbol 12-myristate 13-acetate (PMA) also increased ESE-3 mRNA expression in a dose-dependent manner, and ESE-3 mRNA expression enhanced by PMA was inhibited by PKC inhibitor (bisindolylmaleimide, BIS) and MEK1/2 inhibitor (U0126). These results suggest that ESE-3 might be an important determinant in an inflammation process. In addition, ESE-3 mRNA expression enhanced by PKC activator is regulated through MEK1/2 pathway in HO-1-N-1 cell.