Development and establishment of high performance functional monomers

温和的自酸蚀粘结剂只能使牙本质部分脱矿，在亚微米混合层内的胶原蛋白周围留下羟基磷灰石。这种残留的羟基磷灰石可以作为受体与功能单体进行化学作用，随后除了微机械杂交外，还有助于粘接性能。因此，我们用X射线光电子能谱和原子吸收光谱表征了三种功能单体与人工合成的羟基磷灰石的粘结作用，并用透射电子显微镜表征了其与牙本质的超微结构。1.比较所考察的三种功能单体的化学结合效率，定量测定羟基磷灰石上的碳浓度发现，即使在暴露30分钟后，10-MDP与羟基磷灰石的结合能力也明显强于4-MET。此外，即使10-MDP与羟基磷灰石短暂接触30次，也会产生一个明显的C1S峰，并且峰强度不会随着暴露时间的延长而进一步增加，这有力地表明在临床上合理的使用时间内，10-MDP与羟基磷灰石具有很高的化学结合潜力。2.透射电子显微镜显示，三种自酸蚀粘接剂都形成了一层浅的(0.5-1μm)杂化层。未染色、未脱钙的切片显示，所有系统仅部分脱矿牙本质，在亚微米混合层内的胶原周围留下羟基磷灰石。染色显示典型的杂化层超微结构，由电子透亮的纤维间隙隔开的交叉带状胶原。在混合层的顶层，通常会出现典型的“粗地毯”。3.10-MDP(10-MDPCa)的钙盐在水中的溶解度最低

Mild self-etch adhesives only partially demineralize dentin, leaving hydroxyapatite around collagen within a submicron hybrid layer. This residual hydroxyapatite may serve as receptor for chemical interaction with the functional monomer and subsequently contribute to adhesive performance in addition to micro-mechanical hybridization. We therefore characterized the adhesive interaction of three functional monomers chemically with synthetic hydroxyapatite using X-ray Photoelectron Spectroscopy and Atomic Absorption Spectrophotometry, and ultra-morphologically with dentin using Transmission Electron Microscopy.1.Comparing chemical bonding efficacy of the three functional monomers investigated, quantitative determination of carbon concentration on hydroxyapatite revealed that the bonding potential of 10-MDP to hydroxyapatite is significantly stronger than that of 4-MET, even after 30-min exposure. Moreover, the fact that even a short 30-s exposure of hydroxyapatite to 10-MDP produced a significant C 1s peak and that the peak intensity increased no further by extending the exposure time, strongly suggests that 10-MDP has a high chemical bonding potential to hydroxyapatite within a clinically reasonable application time.2.TEM revealed that all three self-etch adhesives formed a shallow (0.5-1μm) hybrid layer. Unstained, non-demineralized sections revealed that all systems demineralized dentin only partially, leaving hydroxyapatite around collagen within the submicron hybrid layer. Staining disclosed a typical hybrid-layer ultra-structure with cross-banded collagen separated by electron lucent interfibrillar spaces. A typical ‘shag-carpet' often appeared at the hybrid-layer top. Stained, demineralized sections confirmed the acid-resistance of the hybrid layer.3.The calcium salt of 10-MDP (10-MDPCa) was significantly the least soluble in water