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Establishment of an autologous cell transplantion method using mesenchymal stem cells for alveolus bone regeneration.

建立利用间充质干细胞进行牙槽骨再生的自体细胞移植方法。

基本信息

批准号：
16591947
负责人：
FUJISAWA Takuo
金额：
$ 1.98万
依托单位：
OKAYAMA UNIVERSITY
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2005
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16591947/
关键词：
mesenchymal stem cell multilineage differentiation connective tissue growth factor bone regeneration hydroxyapatite tissue engineering cell attachment cell proliferation 血管内皮細胞細胞遊走能 CTGF

项目摘要

The purposes of this research are to investigate the possibility of autologous mesenchymal stem cell transplantion method for alveolus bone regeneration using connective tissue growth factor (CTGF).1. Isolation of human bone marrow cells and cell characterization.Bone marrow cells were isolated from human iliac bone marrow of volunteer. Human bone marrow cells were plated and cultured in DMEM containing 10% FBS. Moreover, STRO-1 expressing cells were identified by immunostaining from culture of passage 9 cells, and these cells showed the multilineage differentiation (osteoblastic and adipogenic).2. Effects of CTGF on stem cells behavior.1) Cell attachment efficiency onto hydroxyapatite disks is enhanced by coating CCN2/CTGF dose-dependently, and CCN2/CTGF activated p38 MAPK signal pathway via αvβ3 integrin.2) CCN2/CTGF significantly increased the cell proliferation dose dependently.3) The migration assay using Chemotaxicell indicated that CCN2/CTGF significantly increased the cell migration.4) CCN2/CTGF did not affect cell differentiation to the osteoblast.5) CCN2/CTGF also enhanced the endothelial cell attachment and proliferation.6) In vivo implantation study indicated that CCN2/CTGF enhance the stem cell survival and induce their migration into the porous hydroxyapatite scaffold.

本研究的目的是探讨利用结缔组织生长因子（CTGF）进行自体间充质干细胞移植方法进行牙槽骨再生的可能性。 1．人骨髓细胞的分离和细胞表征。骨髓细胞分离自志愿者的人髂骨骨髓。将人骨髓细胞铺板并在含有10％FBS的DMEM中培养。此外，通过免疫染色鉴定第9代细胞培养物中表达STRO-1的细胞，这些细胞表现出多向分化（成骨细胞和成脂细胞）。 2． CTGF 对干细胞行为的影响。1) 涂层 CCN2/CTGF 剂量依赖性地增强了细胞在羟基磷灰石盘上的附着效率，并且 CCN2/CTGF 通过 αvβ3 整合素激活 p38 MAPK 信号通路。2) CCN2/CTGF 剂量依赖性地显着增加细胞增殖。3) 使用 Chemotaxicell 的迁移测定表明 CCN2/CTGF 显着增加细胞迁移。4) CCN2/CTGF 不影响细胞分化为成骨细胞。5) CCN2/CTGF 还增强内皮细胞附着和增殖。6) 体内植入研究表明，CCN2/CTGF 增强干细胞存活并诱导其迁移到多孔羟基磷灰石中脚手架。