Development of artifical vascular system with bioabsorbable polymer

生物可吸收聚合物人工血管系统的开发

• 批准号: 16592004
• 负责人: EGUCHI Satoru
• 金额: $ 2.3万
• 依托单位: The University of Tokushima
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16592004/
• 关键词: cell culture; collagen; blood cells; 血球成分

We investigated carrier of the cell culture which we used high culture medium cycloid type culture apparatus for of the oxygen supply efficiency that divided a culture cell and gas saturation culture medium making alveus. We paid attention to a bioabsorbable polymer as carrier to transplant cultured cells to organism and organism material to use for them, biodegradability surgical suture and a collagen thread. As for these material, the collagen thread produced carrier of the configuration which was various to lack intensity of a thread in comparison with floss experimentally. The cellular culture that we used culture apparatus for examined a culture condition and influence of collagen. <methods> Cell carrier produced it experimentally in a simple texture ingredient using biodegradable surgical suture, collagen thread. Cell culture incubated it using the bone marrow which we resected and we measured a blood cell and examined culture medium and influence of collagen. <results> Cell carrier produced a barrel and a flat type experimentally in consideration of various how to weave and characteristics of material. The cell culture was used with culture apparatus and the ingredient of blood cell was measured. As for the erythrocyte and the leucocyte, a tendency to decrease was recognized in a blood cell ingredient in cellular culture, and in most of the haemoglobin was not. In addition, blood platelet accepted increase and showed increase in comparison with control culture. And also the culture using discrete blood constituent for than bone marrow revealed an increase tendency to hematopoietic stem cell. A blood level was equal to or less than 10%, and a tendency to decrease of cell count was seen with a time course, but level of 20%-40% revealed an increase tendency to hematopoietic stem cell. And the increase of an interstitial cell was not accepted.

研究了划分培养细胞的氧供给效率和制作细胞泡的气体饱和度培养基的高培养基摆线式培养装置的细胞培养载体。我们关注的是作为将培养细胞移植到生物体的载体的生物可吸收聚合物以及用于它们的生物体材料、可生物降解的手术缝合线和胶原线。对于这些材料，胶原线通过实验制作了结构不同的载体，与牙线相比，缺乏线的强度。使用培养装置进行的细胞培养，调查了培养条件和胶原蛋白的影响。 <方法> 细胞载体使用可生物降解的手术缝合线、胶原线，以简单的质地成分进行实验生产。使用切下的骨髓进行细胞培养培养，测定血细胞，研究了培养基和胶原蛋白的影响。 <结果> 考虑到各种编织方法和材料特性，细胞载体实验性地生产了筒形和平板形。使用培养装置进行细胞培养，测定血细胞的成分。对于红细胞和白细胞，在细胞培养物中的血细胞成分中发现了减少的趋势，而在大部分血红蛋白中则没有减少的趋势。另外，血小板接受增加并且与对照培养物相比显示出增加。并且使用离散血液成分进行骨髓培养也显示出造血干细胞的增加趋势。血液水平等于或低于10%，随着时间的推移，细胞计数有减少的趋势，但20%-40%的水平显示造血干细胞有增加的趋势。并且不接受间质细胞的增加。