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A basic research on orofacial pain originating from cervical structure: Referred pain

颈椎结构引起的口面部疼痛的基础研究：牵涉痛

基本信息

批准号：
16592025
负责人：
IMAMURA Yoshiki
金额：
$ 1.98万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2004
资助国家：
日本
起止时间：
2004 至 2006
项目状态：
已结题

项目摘要

Patients occasionally present with a complaint of orofacial pain originating from cervical structures. The mechanisms of orofacial pain with cervical origin are not well known. We studied the etiology of this type of pain on a cervical nerve injury model. 6 SD rats underwent two ligation s on C2-C5 cervical nerves followed by section between them (CN group). 6 SD rats received sham-operation (SO group). Another 6 rats received cervical sympathectomy before nerve injury (SE group). Escape behavior was observed before and after operation (POD14) in all rats. Expression of fos-like immunoreactive protein in cells (Fos-LI cells) was observed with tactile stimulation on ipsilateral preauricular skin on POD14. The results were as follows. 1. Cervical nerve injury resulted in allodynia in trigeminal territory. 2. Fos-LI cell expression was observed not only Vi/Vc and Vc/C levels but also middle Vc and C3. 3. Cervical sympathectomy led to no significant behavioral changes. 4. Sympathectomy resulted in significant expression of Fos-LI cells Vi/Vc and Vc/C levels. These results revealed that cervical nerve injury may elicit hypersensitization of secondary neurons in wide area from Vi/Vc to upper cervical spine. This central sensitization may be due to convergence of the trigeminal nerve and cervical nerves. Cervical sympathectomy may modulate signal transduction in nociceptive neurons in uppercervical spine.

患者偶尔会出现源于颈部结构的口面部疼痛的主诉。颈源性口面部疼痛的机制尚不清楚。我们在颈神经损伤模型上研究了这种类型疼痛的病因。6只SD大鼠，C2-C5颈神经结扎两次，然后切断（CN组）。6只SD大鼠行假手术（SO组）。另6只大鼠在神经损伤前切除颈交感神经（SE组）。观察各组大鼠手术前后（POD 14）的逃避行为。在POD 14，触觉刺激同侧耳前皮肤，观察Fos样免疫反应蛋白在细胞（Fos-LI细胞）中的表达。结果如下。1.颈神经损伤导致三叉神经区异常性疼痛。2. Fos-LI细胞不仅在Vi/Vc和Vc/C水平表达，而且在Vc和C3中也有表达。3.颈交感神经切除术没有导致显著的行为变化。4.交感神经切除后，Fos-LI细胞Vi/Vc和Vc/C水平显著升高。提示颈神经损伤可引起Vi/Vc至上颈椎广泛区域的次级神经元超敏反应。这种中枢敏化可能是由于三叉神经和颈神经的会聚。颈交感神经节切除可调节上颈椎伤害性感受神经元的信号转导。