A study on the role of neurotrophines in trigeminal neuropathy and sensory abnormality

神经营养因子在三叉神经病和感觉异常中的作用研究

• 批准号: 13672113
• 负责人: IMAMURA Yoshiki
• 金额: $ 1.73万
• 依托单位: Kyushu Dental College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2001
• 资助国家: 日本
• 起止时间: 2001 至 2002
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-13672113/
• 关键词: Nerve injury; Trigeminal Nerve; Neuropathy; NGF; BDNF

A chronic compression injury to the trigeminal ganglion by an injection of medical silicone via the foramen ovale was induced on seven anesthetized rats (TNP group). The same dissection to the TNP group without injecting silicone was performed on another seven rats (SHM group). Seven rats received cysternal injection of NGF (NGF group) and another seven rats received BDNF (BDNF group). The TNP group and the NGF group showed heat hyperalgesia from fourth post-operative day and mechanical allodynia from seventh post-operative day. However, the SHM and the BDNF groups did not show any significant decrease of thresholds during the time course. These results showed that NGF induces central sensitization by itself and BDNF doesn't have such an effect. In the next study, rats received 200 mg/kg/day or 2000 mg/kg/day of i.p. GSH (NGF-200 group : n-7 and NGF-2000 group : n=7) and 4 mg/kg/day of DEM instead of GSH (NGF-DEM group : n=7) from three days before the NGF injection. Control groups to these groups were prepared by performing sham treatment instead of the NGF injection (SHM-2000 and SHM-DEM groups). NGF-200 and NGF-DEM groups showed decrease of head withdrawal latency from radiant heat stimulation from fourth and seventh post-operative days, respectively. In contrast, NGF-2000, SHM-2000 and SHM-DEM groups did not show any significant behaviors suggesting heat- and mechano-hyperalgesia or allodynia. These results revealed that GSH prevented hyperalgesia by suppressing hypersensitization of the trigeminal nucleus cells that is induced by NGF.

7只麻醉大鼠（TNP组）经卵圆孔注射医用硅胶造成三叉神经节慢性压迫性损伤。另外7只大鼠（SHM组）进行与TNP组相同的解剖，但不注射硅胶。其中7只大鼠接受神经生长因子（NGF）和脑源性神经营养因子（BDNF）的注射。TNP组和NGF组从术后第4天开始出现热痛觉过敏，从术后第7天开始出现机械性痛觉超敏。然而，SHM和BDNF组在时间过程中没有显示出任何显著的阈值降低。这些结果表明，NGF本身具有中枢致敏作用，而BDNF则无此作用。在接下来的研究中，大鼠从注射NGF前三天开始接受200 mg/kg/天或2000 mg/kg/天的腹膜内GSH（NGF-200组：n = 7和NGF-2000组：n=7）和4 mg/kg/天的DEM代替GSH（NGF-DEM组：n=7）。这些组的对照组通过进行假处理而不是注射NGF来制备（SHM-2000和SHM-DEM组）。NGF-200组和NGF-DEM组分别从术后第4天和第7天开始显示出头部从辐射热刺激的撤回潜伏期减少。相比之下，NGF-2000、SHM-2000和SHM-DEM组没有显示出任何表明热和机械痛觉过敏或异常性疼痛的显著行为。这些结果表明，GSH通过抑制由NGF诱导的三叉神经核细胞的超敏化来防止痛觉过敏。

项目成果

Yoshiki Imamura, Eiji Sakamoto, Shunji Shiiba, Masatsugu Iwamoto, Hiroshi Kawahara, Shin-ichi Masumi and Osamu Nakanishi: "Prognosis of prolonged abnormal sensation after dental treatment with quantitative sensory testings"Pain Research. 16(2). 83-89 (200

Yoshiki Imamura、Eiji Sakamoto、Shunji Shiiba、Masatsugu Iwamoto、Hiroshi Kawahara、Shin-ichi Masumi 和 Osamu Nakanishi：“通过定量感觉测试预测牙科治疗后长期异常感觉”疼痛研究。

Yoshiki Imamura, Jeffery P.Okeson: "Chapter 22, Diagnosis of Chronic Orofacial Pain, Management of Pain and Anxiety in the Dental Office, Raymond A. Dionne, James C. Phero and Daniel Becker eds."W. B. Saunders. 418 (2002)

Yoshiki Imamura、Jeffery P.Okeson：“第 22 章，慢性口面部疼痛的诊断、牙科诊所疼痛和焦虑的管理，Raymond A. Dionne、James C. Phero 和 Daniel Becker 编辑。”W.

Yoshiki Imamura, Shunji Shiiba, Eiji Sakamoto, Shin-ichi Masumi and Osamu Nakanishi: "Predicting prognosis of abnormal sensation after trigeminal nerve injury"American Pain Society Bulletin. 12(3). 3-10 (2002)

Yoshiki Imamura、Shunji Shiiba、Eiji Sakamoto、Shin-ichi Masumi 和 Osamu Nakanishi：“预测三叉神经损伤后异常感觉的预后”美国疼痛协会通报。

坂本英治, 椎葉俊司, 今村佳樹, 石川敏三, 鱒見進一, 岩本将嗣, 河原 博, 仲西 修: "ニューロメーターを用いた外傷性三叉神経ニューロパシーの病態評価について"Pain Research. 16(2). 57-62 (2001)

Eiji Sakamoto、Shunji Shiiba、Yoshiki Imamura、Toshizo Ishikawa、Shinichi Masami、Masashi Iwamoto、Hiroshi Kawahara、Osamu Nakanishi：“关于使用神经计进行创伤性三叉神经病的病理学评估”Pain Research 16(2)。 ）

椎葉俊司, 坂本英治, 今村佳樹, 岩本将嗣, 河原 博, 仲西 修: "三叉神経領域におけるCurrent Perception Thresholdの臨床に使用に関する研究"慢性疼痛. 20(1). 126-132 (2001)

Shunji Shiiba、Eiji Sakamoto、Yoshiki Imamura、Masashi Iwamoto、Hiroshi Kawahara、Osamu Nakanishi：“三叉神经区域电流感知阈值的临床应用研究”126-132（2001）。