The development of the vaccine for periodontal disease with using HVJ-envelope

HVJ包膜牙周病疫苗的研制

• 批准号: 16592089
• 负责人: TSURUDA Keiko
• 金额: $ 2.24万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2006
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-16592089/
• 关键词: TFF peptides; HVJ-envelope; Gene transfection; Protein expression; mice; タンパク質発現; マウス; 蛋白発現; 粘液; トレフォイルファクター; TFF3; クローニング

The Trefoil factor family (TFF) peptides are localized on a mucous membrane of digestive organs of a stomach and the bowels. The TFF3 peptide is one of Trefoil factor peptides of developing antibacterial. The TFF3 specifically develops in the small intestine. Recently, the TFF3 was reported to express in submaxillary gland and parotid duct. The aim is to clarify a role of defense for inflammation of a mucous membrane of TFF3 peptide. As for this year of the last year, I examined gene transfection into mice and protein expression with a plasmid vector TFF3 connection pCAGGS, pEGF vector for the gene transfection that made it in the first year.As a result, after TFF3 gene transfection into mice by intraperitoneal injection, I dissected mice on the third day and made a freeze section of the large intestine. The fluorescence that had very weak GFP peptide was observed by fluorescent microscope. When EDTA was supplemented into HVJ-envelope transfection agent, I confirmed the clear fluorescence of GFP protein in a paneth cell neighborhood of a large intestine lamina propria. These results were concluded that protein expression by gene transfection in mice with using HVJ-envelope transfection agent was necessary to enhance the efficacy of gene transfection.in future, it is thought that a vector except a pCAGGS vector namely examination such as the vectors which connected a TFF3 gene with a pEGF vector is necessary. Furthermore, necessity of a device to raise introduction efficiency became clear.

三叶因子家族（TFF）肽定位于胃和肠的消化器官的粘膜上。TFF 3肽是正在开发的抗菌活性三叶因子肽之一。TFF 3特别在小肠中发育。最近有报道称TFF 3在颌下腺和腮腺导管中表达。目的是阐明TFF 3肽的粘膜炎症防御作用。至于去年的这一年，我检查了基因转染到小鼠和蛋白表达与质粒载体TFF 3连接pCAGGS，pEGF载体的基因转染，使它在第一年。结果，TFF 3基因转染到小鼠通过腹腔注射后，我解剖小鼠的第三天，并作出了大肠的冷冻切片。荧光显微镜下观察到荧光很弱的GFP肽。当EDTA被补充到HVJ包膜转染剂中时，我证实了GFP蛋白在大肠固有层的潘氏细胞附近的清晰荧光。这些结果得出结论，使用HVJ-包膜转染剂在小鼠中通过基因转染进行蛋白质表达对于增强基因的功效是必要的transfection.in未来，认为除了pCAGGS载体之外的载体即检测例如将TFF 3基因与pEGF载体连接的载体是必要的。此外，提高导入效率的装置的必要性变得明显。