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In situ and constitutive analysis of peristaltic movement in the ureter

输尿管蠕动运动的原位和本构分析

基本信息

批准号：
17500278
负责人：
YAMASHITA Toshikazu
金额：
$ 2.3万
依托单位：
Nihon University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
2005
资助国家：
日本
起止时间：
2005 至 2006
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-17500278/
关键词：
ureter smooth muscle peristalsis pacemaker

项目摘要

The purpose of this study was to identify pacemaker cells and to perform integrated analysis on mechanisms of the ureteral peristalsis. We developed in situ Ca^<2+> imaging technique of the rat utero-pelvic preparation using a macro zoom microscope. The bright clear view and smooth zooming operation with this technique enabled us to search upstream of Ca^<2+> transient and to identify the pacemaker region easily. Using the same technique we could also successfully record image of di-4-ANEPPS, a voltage-sensitive dye. The initial depolarization occurred at the exactly same place as the Ca^<2+> transient began. With higher magnification, we could observe that not only one cell but several cells increased their intracellular Ca^<2+> concentration simultaneously. Their propagating pathway were slightly different every time. Interestingly spontaneous Ca^<2+> rises in single cells were observed in the other part of renal pelvis and even in the ureter. They are not synchronous to the pristaltic movement and rarely propagated to neighboring cells. In the presence of low concentration of heptanol, a gap junction blocker, the cells forming the pacemaker region lost their synchronism and their function as the pacemaker. These results might suggest that summation of electric activity via gap junctions played an important role in the formation of the pacemaker region.

本研究的目的是鉴定起搏器细胞，并对输尿管蠕动的机制进行综合分析。我们建立了大鼠子宫盆腔制备的原位Ca^<2+>成像技术。该技术具有清晰的视野和平滑的放大操作，使我们能够在Ca^<2+>瞬变上游进行搜索，并容易地识别起搏器区域。使用同样的技术，我们还可以成功地记录di4 - anepps（一种电压敏感染料）的图像。初始去极化发生在Ca^<2+>瞬态开始的同一位置。在放大倍率较高的情况下，我们可以观察到不仅一个细胞，而且多个细胞同时增加了细胞内Ca^<2+>浓度。它们的传播途径每次都略有不同。有趣的是，在肾盂的其他部分，甚至在输尿管也观察到单细胞自发的Ca^<2+>升高。它们与柱体运动不同步，很少传播到邻近的细胞。在低浓度的庚醇（一种间隙连接阻滞剂）存在下，形成起搏器区域的细胞失去了其同步性和起搏器的功能。这些结果可能表明，通过间隙连接的电活动总和在起搏器区域的形成中起重要作用。